Safe Food is paramount
BINDER KBF Constant
Climate Chambers
control temperature and humidity for:
Figure 3 – Synthetic SLE versus diatomaceous earth SLE using DCM as an extraction solvent. Lyso 1: 1-Palmitoyl-2-OH-sn-glycero-phosphocholine (m/z 496–184). Lyso 2: 1-Oleoyl-2-OH-sn-glycero-phosphocholine (m/z 522–184). PC 1: 1-Palmitoyl-2-oleoyl-sn-glycero-phosphocholine (m/z 761–184). PC 2: 1-Stearoyl-2-lindoleoyl-sn-glycero-phosphocholine (m/z 787–184). PC 4: 1-Oleoyl-2-lindoleoyl-sn-glycero-phosphocholine (m/z 784–184).
• Shelf life • Storage parameters • Attributes
www.
stability-test-chamber. com
Figure 4 – Recovery of nine NSAID compounds using synthetic SLE under DCM and EtOAc in DCM extraction conditions.
Conclusion While a reduction in the amount of time spent performing sample preparation can improve total analysis time, analysts must be careful that downstream results and column lifetime are not negatively aff ected as a result of the sample preparation process. Rapid techniques such as protein precipitation are often adopted; however, this technique simply removes pro- teins and does not remove phospholipids, which can cause a huge decrease in HPLC/ UHPLC (ultrahigh-performance liquid chroma- tography) column lifetime. As column lifetime decreases, consumable costs and the amount of time spent changing columns will increase, eff ectively canceling out the time saving ben- efi ts of the quick protein precipitation.
An acceptable compromise to protein precipita- tion, LLE removes proteins, phospholipids and salts from samples and thus improves down- stream results and column lifetime, but can be a cumbersome process and is diffi cult to automate. SLE is gaining popularity because it requires minimal method development and optimization time, follows a simple procedure and is eas- ily automated. Further improvements to the SLE process have resulted in a synthetic SLE sorbent that alleviates the reliance on a natural resource as well as the inherent challenges associated with the diatomaceous earth sorbent.
Erica Pike is the sample preparation brand man- ager at Phenomenex, 411 Madrid Ave., Torrance, Calif. 90501, U.S.A.; tel.: 310-212-0555; e-mail:
ericap@phenomenex.com;
phenomenex.com.
AMERICAN LABORATORY • 21 • MARCH 2015
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