Table 1 – HPLC/MS method parameters for cyclophosphamide analysis


Analytical column: Zorbax Eclipse XDB-C18 (RP); 4.6 × 150 mm, par- ticle size: 5 µm; pore size: 80 Å (Agilent Technologies)


Injection volume: 10 µL Flow rate: 0.75 mL/min


Elution: isocratic, MeOH:water (0.1% HCOOH); 60%/40% (vol/vol) Column temperature: 41 °C Ion source: dual ESI Ion polarity: positive


Figure 1 – Positive pressure unit for automated solid-phase extraction shown with a 96-deep-well plate for cyclophosphamide extraction work- ing with positive pressure.


Drying gas temperature: 330 °C Drying gas flow rate: 10 L/min Nebulizer pressure: 20 psig Capillary: 4000 V Fragmentor: 200 V Collision energy: 65 V Range: 100–3000 m/z


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