Freeze-Thaw Stability Stability studies of IBP, IBP 2-hydroxyethyl ester, and codrug in both human plasma and rat liver homogenate samples indicated feasibility of storage in the freezer at -15°C for at least 2 months without signification degradation.
APPLICABILITY The described method was applied successfully to determine codrug’s solubility in aqueous and organic solvents and partition coefficient, and to evaluate stability of codrug and IBP 2-hydroxyethyl ester in aqueous medium of 0.05 M phosphate buffer (pH 1.2, 6.8, and 7.4) at 70, 80, and 90°C. The method was also implemented for in vitro enzymatic hydrolysis of IBP and nicotinic acid codrug and IBP 2-hydroxyethyl ester in human plasma and rat liver homogenate; enabling hydrolysis
FIGURE 3: Degradation of ibuprofen- nicotinic acid codrug and ibuprofen 2-hydroxyethyl ester in 20% rat liver homogenate
Codrug 0.3 0.25 0.2 0.15 0.1 0.05 0 0 10 20 Time (mins) 30 40 50
REFERENCES References available on request (
magazine@informa.com)
Ibuprofen Ester Ibuprofen
evaluation of codrug and two parent drugs (IBP and IBP 2-hydroxyethyl ester). It was found that codrug and IBP 2-hydroxyethyl ester exhibited pseudo-first-order kinetics and had a half-life of 0.177 and 0.0569 min-1 in rat liver homogenate (see figure 3).
CONCLUSION A simple RP-HPLC method has been developed and completely validated for the simultaneous determination of IBP, IBP 2-hydroxyethyl ester, and IBP 2-hydroxyethyl ester - nicotinic acid codrug. This method is rapid with a simple binary mobile phase that needs no adjustment to accommodate the differences in retention times of all the analytes. This method was successfully used in the determination of physicochemical properties such as solubility and partition coefficient of IBP 2-hydroxyethyl ester - nicotinic acid codrug. It was also used for evaluation of in vitro chemical stability and enzymatic hydrolysis of IBP 2-hydroxyethyl ester - nicotinic acid codrug and IBP 2-hydroxyethyl ester.
MAGAZINE
ACKNOWLEDGMENTS This work is a part of Fatima Zaid Abu Zanat MSc dissertation. It has been funded by a grant from the Deanship of Research at the Jordan University of Science and Technology (JUST), Irbid - Jordan. We would like to thank Dr Amjad Qandil and Prof Bassam Tashtoush at JUST for supervising our MSc dissertation. Our thanks also extend to Mr. Eyad Hamzeh from Pharmaceutical Research Center at JUST, Irbid – Jordan for their continuous co-operation as well as Jordanian Pharmaceutical Manufacturing, Naour – Jordan for generously providing IBP.
042 MEDLAB MAGAZINE ISSUE 1 2012
Cone (mmol)
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