Screening
review for publication). In addition to ion channel solutions and consumable array plates, Librede will offer a benchtop workstation for scientific studies and an automated parallel instrument for increased throughput.
Figure 23: Librede’s array plate used for cell-free electrophysiological ion channel screening
compatible with SBS standard 96- and 384-well formats, with 1536-well format in development. The number of measured ion channels can be con- trolled as desired to obtain single channel currents for detailed biophysical studies or the measure- ment of thousands of channels, equivalent to whole cell patch clamp for drug screening. Measurement solutions may be easily exchanged during experimentation, reducing screening time and cost while increasing throughput. Librede’s platform has been validated with a number of mammalian ion channels including hERG with measured IC50/EC50 matching published values (in
Figure 24
Molecular Devices’ IonWorks Barracuda instrument is a high-throughput screening
system for the measurement of ligand- or voltage-gated ion channels
Molecular Devices (
www.moleculardevices.com) offers instrumentation for ion channel research ranging from fully automated high throughput screening systems to amplifiers for traditional low- throughput manual patch clamp setups. IonWorks instruments are typically found in laboratories requiring the screening of large compound libraries. They have historically offered the highest through- put and lowest running costs of commercially avail- able systems. The IonWorks Barracuda system, released in 2010, screens both voltage- and ligand- gated channels and generates a throughput of 1,100 and >6,000 data points per hour for screening and compound profiling, respectively. The IonWorks Quattro system, released in 2005, introduced the Population Patch Clamp (PPC) recording technique to ion channel screening. This breakthrough tech- nology boosted the throughput of IonWorks systems four-fold and allowed for the first time ion channel screening of large libraries up to several hundred thousand compounds. PPC well-to-well data consis- tency allowed the generation of novel assays which resulted in multiple peer-reviewed publications. The PatchXpress system also offered by Molecular Devices is a 16-channel automated patch clamp sys- tem with data quality identically comparable to tra- ditional patch-clamp set-ups. Released in 2003 it has proven to be a workhorse in many of our large pharmaceutical, biotech and CRO customer’s labs. We also offer Axon CNS amplifiers that are used on more traditional patch clamp set-ups than any other brand. Overall Molecular Devices has the largest presence of instrumentation for ion channel research ranging from screening and safety laboratories to academic research (Figure 24).
Nanion Technologies (
www.nanion.de) is a provider of automated patch clamp platforms sup- porting high quality electrophysiology recordings. Starting as a spin-off from the Center of Nanoscience, LMU in Munich, Germany, Nanion has grown into a profitable company with branch- es in the USA and China. The Port-a-Patch – “the world’s smallest patch clamp rig” – was launched in 2003 and is used in more than 200 research lab- oratories around the globe. It reduces an entire patch clamp set up to something you can hold in your hand, however still providing high quality, giga-seal recordings. The Patchliner followed in
58 Drug Discovery World Fall 2011
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