Screening
getting commonly used for the drug safety pharma- cology and applying the ESCs /iPSCs is a consequent trend. The newly developed FDSS/uCELL is a com- pact, simple to use, affordable system, yet equipped with the simultaneous dispensing head, active tip wash and high speed and high sensitivity camera. The Hamamatsu FDSS/uCELL and the assay system are suitable for miniaturisation to save both time and cost from early to late stage drug discovery (Figure 22).
Librede (
www.librede.com) is developing a plat- form for cell-free electrophysiological ion channel screening. Librede’s technology measures ion chan- nels in artificial membranes made from solutions derived directly from simply processed source cells, eliminating the need for cell incubation and han- dling by the end user. Electrophysiological meas- urements of ion channels are comparable to patch clamp and automated patch clamp but with 10- 100 G seal resistance and high yield. In addition to ion channels obtained from common cell mem- branes (eg HEK, CHO), measurement of ion chan-
Figure 22: Ca2+ oscillations in cardiomyctes detected by Hamamatsu’s FDSS/uCELL
nels from difficult to patch primary cells or sub-cel- lular organelles is straightforward. Ion channels are measured in inexpensive consumable array plates that allow simultaneous optical and electri- cal measurement. The array plates (Figure 23) are
New Cell-based Assay Plate Reader, FDSS/µCELL
The FDSS/µCELL is a new compact and cost-effective plate reader for fluorescent kinetic cell-based assays.
Features 96/384 well dispensing All wells dispensed simultaneously Fast data acquisition -
uses Hamamatsu CCD camera technology
Whole plate imaging Agonist/Antagonist assays with tip washing Compact Simple to use
Applications Intracellular ion measurement (Ca2+, fluo-3/-4) Membrane potential (FMP)
PHOTON IS OUR BUSINESS
www.fdssdrug.com
Freephone: Europe 00 800 800 800 88, USA 1-800 524 0504
Drug Discovery World Fall 2011
57
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92