Cell Culture
standard RCCS that will allow harvesting of the stem cells without disturbing the bio-artificial niche. The goal of this project is to mimic the in vivo production of hematopoietic stem cells in a scalable bioreactor system that can reliably expand these cells for therapeutic applications (Figure 23).
Figure 24: The RAFT (Real Architecture for 3D Tissue) system from The Automation Partnership forms tissues in less than an hour by controlling compression and incubation of collagen-based gels seeded with cells. The system, which uses a SBS standard 24-well plate format can be filled manually and will fit into a standard laminar flow hood to prevent contamination of tissue constructs
blood stem cells. The bone marrow stromal cells (MSCs) are first seeded with the scaffolds in the RCCS and allowed to proliferate to confluence. The umbilical cord stem cells are subsequently injected into the bioreactor and induced to home to the scaffolds by chemoattractive signalling from the MSCs. Stem cells will be detached from the scaffolds by mobilising agents currently used in vivo. Synthecon has designed a modification of its
RAFT (Real Architecture for 3D Tissue) is a new sys- tem for scientists to create consistent, well defined 3D tissues in a convenient, simple-to-use format. It has been developed by The Automation Partnership (TAP) (
www.automationpartnership.com) in collab- oration with leading tissue engineering academics and uses a novel, patented technology for making 3D collagen tissue constructs rapidly (in vivo stem cell microenvironment, and then culture a different cell type on the surface. RAFT allows complex mul- tilayer tissues to be formed – with different cell types in each layer – and cells to be co-cultured in a well controlled way. The resulting biomimetic tissues, made from fibrillar collagen (the main component of extracellular matrix), are strong, transparent and 50- 100µm thick. TAP has developed a workstation and consumables to automate and scale up this 3D tissue production process, enabling up to 24 tissues to be made in parallel. Tissues are made either in the wells of a 24-well plate; on permeable membrane inserts for barrier assays or cultured at an air/liquid inter- face, for example to form stratified epithelia. The tis- sue remains in the same well from its creation until the end of the experiment and can be analysed using standard techniques. TAP will be unveiling RAFT at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) Conference, on June 13-17, 2010 in Galway, Ireland (Figure 24).
Figure 25: Tissue Growth Technologies’ DermiGen™ Mechanical Stimulation Bioreactor System
38
The DermiGen™ Mechanical Bioreactor, manufac- tured by Tissue Growth Technologies (www.tissue-
growth.com), imparts static and oscillatory strain to a skin-like sample in a unique air/media inter- face. The device may be used to stimulate the growth of tissue engineered skin or to act as a test bed for drug and cosmetic development. Featuring a linear motor driven mechanical stimulator, the DermiGen applies mechanical strain using opera- tor defined parameters. The bioreactor’s unique chamber design provides nutrient media perfusion
Drug Discovery World Summer 2010
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