Cell Culture
tion in 3D. QGel™ matrix products are available in sterile, ready-to-use vials manufactured under strict GMP conditions.
Figure 20: Tissue Equivalent Culture in the RealBio™ D4 Culture System
that define the biological and biochemical charac- teristics of its matrix are like building-blocks that allow for QGel to be bioengineered on a molecular level. This building-block modularity gives QGel™ advantages over natural ‘gold standard’ matrices, where ‘what you get’ depends on the origin of the organism from which it was derived. The ability to synthesise and bioengineer QGel™ offers scientists an exclusive possibility to adapt these biomimetic 3D cell culture models to specific cell types and to investigate different aspects of cell-matrix interac-
RealBio™ Technology (
www.realbiotechnology.com) has introduced the D4 Culture System, a revolution- ary tool for growing cells and tissues in a setting that closely mimics the in vivo environment. The RealBio™ D4 Culture System represents an exponen- tial improvement over current 2D and 3D cell culture technologies because it supports mixed-cell popula- tions in varied microenvironments throughout an open scaffold. As a result, tissues and cells cultured in the D4 Culture System arrange as in vivo and demon- strate natural function. In addition, cells cultured in the D4 system exhibit migration throughout and out of the tissue mass, just as cells naturally migrate in vivo. The D4 Culture System also provides researchers with unprecedented control of the culture environment, including control of nutrient and gas gradients across the cultured tissue mass, by decou- pling the supply of nutrient media and metabolic gasses. The D4 Culture System was originally devel- oped with a $5 million grant from the US military and perfected within the RealBio™ Technology laborato- ry in Kalamazoo, Michigan. The patented D4 System is a valuable tool for studying various structured tis- sues (including polar tissues) and has demonstrated the ability to support the growth and maintenance of human bone marrow for longer than one year with daily harvests of a mixed-cell population that includes early progenitor stem cells. Mixed-cell populations harvested from long-term cultures maintained in the D4 system exhibit similar CD marker profiles to the initial seed material thereby demonstrating that the RealBio™ D4 Culture System minimises the selective pressures observed in other culture systems (Figure 20).
Figure 21: RegeneMed Liver3 and RegeneTOX product line for safety and efficacy assessment of new chemical entities
36
RegeneMed (
www.regenemed.com) provides 3D human and animal tissue cultures in multiwell plates and specialised bioreactors to serve as more physiologically relevant replacements to current industry-standard animal and cell-based tests that are often not predictive of the human response to toxic compounds. Liver3, 3D liver co-culture, sus- tains tissue function for months, providing off-the- shelf available, reproducible, reusable, prediction of metabolism, toxicity and efficacy measures for new drug candidates, chemical entities and con- sumer products. The long-term tissue-specific func- tion enables pre-characterised tissues from fresh and cryopreserved hepatocytes for in vitro assess- ment of previously unattainable endpoints such as bioavailability, drug-drug interactions and chronic
Drug Discovery World Summer 2010
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