chromAtogrAphy/spEctroscopy 31
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Fig. 4. The system redirects the light path from the microsample
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pedestal mechanism to the cuvette holder.
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the least amount genetic material derived from precious
Pall’s vast filtration, separation
medical samples, NanoDrop technology is proving to be
andpurification product portfolio,
an important tool for quantifying biomolecules.
product knowledge and technical
Although the sample retention system represents
know-how enable us to deliver
a fundamental paradigm shift away from traditional
products and services that
containment devices, conventional devices do have
truly make a difference in our
their benefits. Temperature controlled experiments and
customers’ work. And NOW,
samples that are volatile lend themselves to traditional
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containment devices. The NanoDrop 2000c combines
both the microsample retention system with a traditional
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cuvette mechanism (Fig. 4). The light path is redirected
Technical Support team for a
from the vertical orientation of the sample retention
proactive response that is fast and
system to the horizontal orientation of the cuvette holder
particularly tuned in to your unique
mechanism. The dual arrangement allows flexibility
lab application requirements –
to take advantage of the broad dynamic range and
all day long!
microvolume capability of pedestal measurements as well
as methods in which traditional cuvette measurements
may be more suitable.
Spectrophotometers have long been used to estimate
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the growth stage of a microbial cell culture by using the
your FREE
empirical observation that light scattering caused by the
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cell culture turbidity can be measured. Although this
“Discoveries”
observation is empirical rather than true absorbance,
Poster, by
most scientists are familiar with the absorbance values
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using a standard one centimeter cuvette and closely
Lab@pall.com.
correlate those values with the growth stage of their
cultures. The dual capability of the NanoDrop 2000c
allows investigators to measure cell cultures in vivo, then
subsequently measure extracted biomolecules using
the microsample pedestal method. Due to the potential
evaporation of microvolume samples, kinetic studies
and other assays that also require several time point
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readings are more amenable to cuvette measurements.
Give us a call.
The NanoDrop 2000c cuvette mechanism contains a
1.800.521.1520
temperature control option for common time point assays
(USA and Canada)
such as those used for enzymatic kinetics studies. u
(+)800.7255.5433
Enter 31A or ✔ at www.scientistlive.com/elab
(Outside USA and Canada)
LabCustomerSupport@pall.com
Philippe Desjardins is scientific marketing manager for
NanoDrop instruments at Thermo Fisher Scientific, Wilmington,
© 2007 Pall Corporation. Pall and are trademarks of
Pall Corporation. ® indicates a trademark in the USA. GN07.1635
USA. www.thermofisher.com
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