CHROMATOGRAPHY/SPECTROSCOPY 31


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Fig. 4. The system redirects the light path from the microsample pedestal mechanism to the cuvette holder.


the least amount genetic material derived from precious medical samples, NanoDrop technology is proving to be an important tool for quantifying biomolecules. Although the sample retention system represents a fundamental paradigm shift away from traditional containment devices, conventional devices do have their benefits. Temperature controlled experiments and samples that are volatile lend themselves to traditional containment devices. The NanoDrop 2000c combines both the microsample retention system with a traditional cuvette mechanism (Fig. 4). The light path is redirected from the vertical orientation of the sample retention system to the horizontal orientation of the cuvette holder mechanism. The dual arrangement allows flexibility to take advantage of the broad dynamic range and microvolume capability of pedestal measurements as well as methods in which traditional cuvette measurements may be more suitable. Spectrophotometers have long been used to estimate


the growth stage of a microbial cell culture by using the empirical observation that light scattering caused by the cell culture turbidity can be measured. Although this observation is empirical rather than true absorbance, most scientists are familiar with the absorbance values using a standard one centimeter cuvette and closely correlate those values with the growth stage of their cultures. The dual capability of the NanoDrop 2000c allows investigators to measure cell cultures in vivo, then subsequently measure extracted biomolecules using the microsample pedestal method. Due to the potential evaporation of microvolume samples, kinetic studies and other assays that also require several time point readings are more amenable to cuvette measurements. The NanoDrop 2000c cuvette mechanism contains a temperature control option for common time point assays such as those used for enzymatic kinetics studies. u


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Philippe Desjardins is scientific marketing manager for NanoDrop instruments at Thermo Fisher Scientific, Wilmington, USA. www.thermofisher.com


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