chromatography/spectroscopy 35
Non-linear optical New chromatography column chemistries
materials hold promise
for better imaging
p
all Life sciences has launched a feature luer inlet and outlet for convenient
full palette of scaleable acrosep use with syringe, pump or automated
1ml columns with ion exchange, affinity, chromatography system.
W
hen photographers zoom in on an and mixed-mode
u high binding
object to see it better, they lose chromatography
capacities, fast
the wide-angle perspective – they are chemistries. pall
flow rates. By
forced to trade off 'big picture' context acrosep pre-packed
tailoring attributes
for detail. But now an imaging method chromatography
such as chemistry,
developed by princeton researchers could columns are ideal
pore size, and
lead to lenses that show all parts of the for preparatory
sorbent diameter
scene at once in the same high detail. the protein purification
to applications, pall
method could help build more powerful research. these
resins and columns
microscopes and other optical devices. columns can also
exhibit the highest
cameras and other optical devices – be utilised as an
performance
including the human eye – are limited by efficient screening
characteristics.
the amount of light that they can collect tool for best
u User-friendly
through their lens openings, or apertures. chromatography
column design.
In order for a light ray to be recorded, it conditions prior
colour coded
has to pass through the lens and reach to process
and labelled by
the device's 'detector' – such as the eye's development.
chemistry type. the
retina or a digital camera's detector. pall acrosep
acrosep columns
But many light rays never make it to the chromatography columns offer:
feature a hexagonal collar so columns will
detector, either because they are too u true scaleability. pall offers the
not unexpectedly roll off lab surface. u
weak, or because they are deflected. same resin chemistry for small-scale
enter 35 or ✔ at www.scientistlive.com/elab
this problem is particularly acute discovery through to biopharmaceutical
with details that are smaller than the manufacturing. Pall Life Sciences is based in East Hills,
wavelength of light. (each colour of u Versatility. pall acrosep columns NY, USA. www.pall.com/acrosep
light has a distinct wavelength – green,
for instance, has a wavelength of 530
nanometres, roughly the size of a typical
mass spectrometric comparison and evaulation
bacterium's internal structure.) Light
rays from such tiny features fade before
of gc-ms and Lc-ms analyses
they reach the lens. to capture these
rays, devices have to probe very near the a
frequent task in an analytical be capable of finding component
laboratory is the comparison of two spectra even if coelutions and variable
surface of the object, and scan it point- samples. real life samples with complex background is present. It must be possible
by-point, stitching together a full image. matrices have complex chromatograms to compare the two analyses based on
the new method addresses the that make the evaluation for presence of retention index scaling and on absolute
shortcomings of small apertures by contamination or residual components a intensities.
taking advantage of the properties of difficult and time consuming task. the spectral comparison must reliably
substances called non-linear optical the compare tool recently developed recognize that a spectrum is contained
materials. for the mass spectra evaluation software in the comparison spectrum even if
the core component, a non-linear package massLib finds both qualitative not readily visible. massLib’s sIscom
wave mixer, is a rectangular pill-sized and quantitative differences between two algorithm (search for Identical or similar
crystal of a material called strontium gc-ms or Lc-ms analyses and presents compounds) is better suited for this task
barium niobate. the researchers placed the results graphically and in tabular than algorithms that are optimised for
the object to be imaged on one side of the form. determination of spectral identity.
crystal and image-capturing equipment the only prerequisite is that the In addition, library searching must
on the other. they tested the system by two analyses have been acquired be available to identify the components
obtaining images of various objects, under chromatographically comparable where differences are found. and finally,
including a chart developed by the air conditions. the comparison must run autonomously
Force that is widely used to calibrate For an efficient and successful and document its results. u
optical devices. u analyses compare tool several features
enter 35a or ✔ at www.scientistlive.com/elab
are required which all are provided in the
For more information, visit massLib software. MSP KOFEL is based in Zollikofen,
www.princeton.edu a peak finder algorithm must Switzerland. www.msp.ch
www.scientistlive.com
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