Drug Discovery

Figure 3 Xevo G2-XS QTOf

Quadrupole Time-of-Flight Mass Spectrometry. For

scientists who need to identify, quantify and confirm the

broadest range of compounds in the most complex and challenging samples

The Waters Xevo TQ-S micro triple quadrupole

mass spectrometer is a compact instrument that is designed for reproducible low-level analyte detec- tion, but with a minimal laboratory footprint. This small-but-mighty system is capable of delivering consistent quantitative performance with a wide dynamic range. The instrument’s next generation T-Wave™ collision cell permits acquisition of 500 multiple reaction monitoring channels per second, while minimising cross-talk and maintaining inten- sity. The increased MS full scan speed capability of up to 20,000 Da per second reduces the impact on the duty cycle when switching between full scan and MS/MS acquisition. The Thermo Fisher Scientific Orbitrap™ instru-

References 1 Campbell, JL, Le Blanc, JY and Kibbey, RG. Differential mobility spectrometry: A valuable technology for analyzing challenging biological samples. Bioanalysis (2015). doi:10.4155/bio.15.14. 2 Pioch, M, Bunz, SC and Neusüß, C. Capillary electrophoresis/mass spectrometry relevant to pharmaceutical and biotechnological applications. Electrophoresis (2012). doi:10.1002/elps.201200030.

widths, at rates of up to 200 per cycle, to achieve better specificity even in complex samples. Combining capillary electrophoresis with electro-

spray ionisation in a single process, the SCIEX CESI 8000 Plus ESI-MS capillary electrophoresis system is designed for rapid analysis of biopharmaceutical, proteomics and metabolomics samples. The system enables researchers to achieve reliable and sensitive separation of intact mAbs and proteoforms with on- line MS detection, eliminating the need for pre-frac- tionation and reinjection. The system also allows the study of molecular interactions and structure of pro- teins and peptides under native conditions. Waters also supplies a broad range of technologies

for high resolution mass analysis. Its Xevo G2-XS QTOF instrument is specifically designed to help researchers achieve confident analyses in even the most challenging of samples. With class-leading quantitative sensitivity, as well as exceptional mass accuracy, dynamic range and speed, thanks to the use of QuanTof™ technology, the system offers superior quantitative performance, even in the presence of interferences from complex matrix components.


ments are delivering exceptional performance when it comes to high resolution biopharmaceuti- cal analysis. The Thermo Scientific™ Q Exactive™ HF-X Hybrid Quadrupole-Orbitrap mass spec- trometer incorporates a high capacity transfer tube for maximum ion loading, and an electrodynamic ion funnel capable of accommodating and trans- mitting ions over a broad mass range. The system also incorporates a high-field Orbitrap™ mass analyser for the rapid identification and analysis of peptides, label-free and tandem mass tag-based (TMT) quantitation, top-down proteomics analy- ses, sophisticated data-dependent acquisition (DDA) and data-independent acquisition (DIA), and dynamic retention time parallel reaction mon- itoring (PRM). Combined with the powerful Thermo Scientific™ Q Exactive™ BioPharma option, the system is capable of supporting highly sensitive biotherapeutic characterisation – from peptide mapping to the analysis of intact proteins under native conditions. Some of the most recent Orbitrap-based instru-

ments, such as the Thermo Scientific™ Q Exactive™ UHMR Hybrid Quadrupole- Orbitrap™ mass spectrometer, also offer enriched sensitivity in the high mass range through their novel in-source trapping. This innovation is enabling improved intact protein transmission in the gas phase and allows researchers to fine-tune protein desolvation (such as in the removal of detergent micelles) and perform consequent frag- mentation of protein drugs, drug targets and pro- tein-ligand complexes in their native state.

The future of the field Improvements in sensitivity, precision and accura- cy of instruments for research purposes have large- ly driven the remarkable advances in instrument design that have taken place over the last decade. However, with biotherapeutics set to play an even

Drug Discovery World Summer 2018

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