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Microscopy, Microtechnology & Image Analysis


Super-resolution microscopy across diverse applications


Zeiss, a pioneer in microscopy solutions, is introducing an innovative addition to its product portfolio with the Zeiss Lattice SIM product family. This development aims to harness the complete potential of structured illumination microscopy (SIM), expanding the capabilities beyond the established super-resolution microscope, Zeiss Elyra 7.


Comprising two new members, Zeiss Lattice SIM 3 and Zeiss Lattice SIM 5, this product family uses structured illumination to overcome the physical resolution limitations of light microscopy. Each system is designed to cater to specifi c fi elds of application, broadening access to super-resolution imaging across diverse domains.


In contrast to conventional structured illumination methods, Zeiss Lattice SIM employs a lattice spot pattern, enhancing imaging speed, contrast, and minimising light exposure. This distinctive approach makes Lattice SIM particularly advantageous for live cell imaging. Additionally, the microscopes in the Zeiss Lattice SIM family integrate SIM Apotome technology, facilitating fast, high-resolution optical sectioning of larger samples such as cell culture models, organoids, and developing organisms. The innovative SIM² image reconstruction algorithm further doubles the SIM resolution.


Addressing unique application needs, Zeiss Lattice SIM 3 caters to multicellular organisms and tissue sections, excelling in


fast optical sectioning with superior quality, large fi elds of view, near-isotropic resolution, and gentle super-resolution imaging. Zeiss Lattice SIM 5 is optimised for single-cell imaging, capturing subcellular structures and their rapid dynamics with outstanding super-resolution down to 60 nm in both living and fi xed cells.


Zeiss Elyra 7 integrates various microscopy techniques such as Lattice SIM², SIM² Apotome, single-molecule localisation microscopy (SMLM), and total internal refl ection fl uorescence (TIRF). This comprehensive approach allows users to combine techniques for deeper insights into specimens and correlation of acquired data, particularly focusing on SMLM for molecular-level resolution.


With the Zeiss Lattice SIM product family, researchers gain precision in addressing specifi c experimental requirements, balancing sample size, imaging speed, and super-resolution capabilities. These cutting-edge imaging tools open new avenues for discoveries in organoid research, cell biology, developmental biology, neuroscience, immunology, and pharmaceutical research.


More information online: ilmt.co/PL/RBlM 61819pr@reply-direct.com


Innovative microscope for simultaneous FTIR and Raman spectroscopy


Shimadzu, a global leader in analytical instrumentation, introduces the ground-breaking AIRsight FTIR Raman microscope, the world’s first and only instrument capable of seamlessly performing both FTIR and Raman spectroscopy. This single, highly efficient instrument streamlines processes and offers diverse applications in industries such as chemical, electrical, electronic, machinery, transportation, and microplastics research.


The synergy of Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy in a single instrument enhances the efficiency of analytical techniques. AIRsight, with its ability to acquire both spectra from the same position using an extremely small spot, delivers crucial complementary information for a comprehensive analysis.


Key features include the efficient gathering of data on both organic and inorganic substances, simultaneous acquisition of FTIR and Raman spectra without sample relocation, and seamless switching between measurement modes using the AMSolution control software. Equipped with Shimadzu’s proprietary wide-view camera, infrared microscope camera, and Raman-use objective lens, the AIRsight provides high-quality imaging and precise observations. The AMSolution control software further enables length measurements, particularly useful for analysing microplastic particles.


AIRsight marks a significant leap in analytical instrumentation, replacing the need for separate FTIR and Raman microscopes with an all-in-one solution. Shimadzu continues its commitment to delivering superior instruments, making processes faster, more compact, and user-friendly.


More information online: ilmt.co/PL/kANd 61631pr@reply-direct.com


High-resolution InGaAs camera with small pixels and low readout noise


The new C16741-40U InGaAs camera from Hamamatsu Photonics showcases an SXGA resolution of 1280 x 1024 pixels and an extended sensitivity range spanning from 400 nm to 1700 nm, encompassing both visible and short-wavelength infrared regions. Boasting 5 μm pixels, it excels in capturing high- resolution images with an exceptional signal-to-noise ratio, attributed to its minimised readout noise.


Furthermore, the camera integrates a Peltier element for efficient sensor cooling and temperature stabilisation. The USB 3.1 Gen 1 interface port facilitates 12-bit image acquisition and exposure time adjustment, contributing to its overall versatility and performance.


The key features of this new InGaAs Camera - high resolution, small pixel, and low readout noise significantly enhance the functionality of SWIR cameras across various applications from food and plastic sorting to silicon wafer inspection, laser beam alignment, and solar cell evaluation.


Any questions regarding the new InGaAs Camera C16741-40U, please contact Hamamatsu Photonics. More information online: ilmt.co/PL/6Kpl


62153pr@reply-direct.com


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