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May/June 2011 Foreword
Welcome to the fourteenth edition of Chromatography Today, with the main theme for this edition being Hydrophilic Interaction Liquid Chromatography (HILIC).
In the excellent opening article “HILIC after the Hype” by Tobias Jonsson, Petrus Hemstrom and co authors review the rise of HILIC and look to predict the future of this separation technology. The paper states that it is currently seen as the fastest growing HPLC separation mode, being used by almost 20% of all HPLC instrument owners.
With its often unique selectivity it is now being used in all major application areas – Metabolomics, Clinical Analysis, Food, Environment and Biomolecules -offering separations for compounds which have proved difficult to retain on conventional HPLC columns and a simpler alternative to conventional ion-pair chromatography.
Two of the other papers in this edition highlight the potential of HILIC separations, Marc Barber, looks at the separation of Dextran’s using a single HILIC column coupled to a Charged Aerosol Detector (CAD). The benefit of such an approach is compared with the current European Pharmacopeia (EP) method which can take in excess of 500 minutes. The method developed can now be performed in less than thirteen minutes and is shown capable of providing the necessary molecular mass distributions.
The second paper by Wen Jiang et al looks at the separation of model aminoglycosides with a zwitterionic stationary phase and mass spectrometry detection. Aminoglycosides are commonly used as bactericidal antibiotics and being very hydrophilic and positively charged, are very difficult to separate by reverse phase chromatography unless an ion-pair reagent or pre column derivatisation is used. HILIC is shown to provide good retention and separation of the aminoglycosides and with the coupling to a single quadrupole ESI-MS instrument offers great potential for low level residue analysis in a variety of sample matrices.
Moving away from HILIC but staying with the theme of chromatographic separation Paul Ferguson – the current Chromatographic Society Vice President, along with one of his Pfizer colleagues Ronan Huet describe their work on the correlation of analyte retention in organic and inorganic phases. As we all know organic mobile phase additives which allow compatibility with mass- spectrometric detection often give high background UV absorbance – reducing the ability for low level detection. In addition they often offer little buffering capacity and this can lead to variability in HPLC retention times. This paper looks at the correlations between the common organic buffers and their potassium phosphate alternatives to establish if methods developed with MS compatible phases could be transferred directly to appropriate phosphate modified phases, when increased sensitivity for impurity detection is required.
An alternative to HPLC is considered in the article by Keith Freebairn who describes the potential of Countercurrent Chromatography (CCC) and the work of the GSK, Pfizer and Brunel University Consortium. The paper provides a brief overview of the current status of CCC and then goes onto describe the key benefits now being offered by the new generation of high- performance countercurrent chromatography instruments. It also describes the potential of CCC to address a variety of purification challenges including isomer separation, recovery of products from mother liquors, and purification of crude reaction products.
Alan Handley - President of the Chromatographic Society
The final technical paper offers an insight into a potential new universal detection technology. Eddie Goodall describes the concept of “water condensation nucleation” and the new instrument based on this technology from Quant Technologies and now being distributed by Dorton Analytical Ltd in the UK.
The Nano Quantity Analyte Detector (NQAD) unlike other evaporative detectors is not dependent on mobile phase composition and offers, as it name suggests, high sensitivity universal detection for HPLC, UHPLC and even SFC systems.
Also Included in this edition is an interview with Howard Hill from HLS here he talks about the drivers causing current changes in the global pharmaceutical industry, the analysis of Biopharmaceuticals and the role of CRO’s .He also discusses his involvement with one of the few international separation meetings held in the UK – “The Reid International BioForum” which is shortly to be held at the University of Surrey.
As usual the second part of the “Foreword” allows me to report on the Chromatographic Society. Our Spring Meeting “Advances in Separation Science” at Novartis provided to be very successfully and attracted a large number of delegates and students (supported by the Chrom Soc bursary scheme).
Thanks go to the organising committee, Novartis and the many instrument companies who helped sponsor the event.
As mention in Howard Hill's interview the next Chrom Soc meeting is “The Reid International BioForum” on the 4th-7th July (see Chrom Soc Website) for details.
Also advertised in this edition is Chirality 2011 held in Liverpool – July
10-13th.Here The Chromatographic Society will host two sessions – one on advances in chiral chromatographic techniques and the other, on issues associated with Chiral Bioanalysis (
www.liv.ac.uk/chirality-2011).
The Society is also planning to hold a meeting on Advances in Gas Chromatography on the 9th of November at Runcorn. This meeting and exhibition has proved to be very popular over the years as it is now one of the few meetings dedicated to GC separations. Further details at
alan.handley@lgc.co.uk.
And finally we are still open to nominations for “The Chromatographic Societies Student Awards”, and the Societies Martin and Jubilee medals – see website for details.
http://www.chromsoc.com.
Happy separations Alan.
Next Issue - Chip-based Separations To be included call us on +44(0)1727 855574 or
info@chromatographytoday.com
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