25 APPLICATION NOTE
Does the choice of UHPLC or Core-Shell confuse you ?
Figure 4. Melamine Contamination
Why not choose the best of both ? Fortis™
Technologies 1.7µm UHPLC Columns Figure 5. Selectivity of complex metabolite mixture. Data courtesy of ScotMet:Glasgow
basic analytes when the unbonded silica substrate is employed. The theory is that the silanols are now freely accessible to interact with the basic analytes instead of any steric hindrance compromising the peak shape.
This improved peak shape for basic analytes is a major advantage with HILIC silica phases, leading ultimately to an improvement in sensitivity.
The bare silica version of the Fortis particles have also recently been shown to exhibit excellent peak shape selectivity and resolution in SFC mode. This will be a major issue to those wishing to asses the suitability of “green techniques” within their laboratory.
Conclusion
In this application note we have shown the mechanisms and considerations that are important to those requiring the HILIC method of chromatography in order to retain the more polar analytes that can cause issues in retention and separation. The correct use of a HILIC column can provide additional selectivity and resolution, especially for hydrophilic analytes. As the understanding of HILIC mechanisms increases then the ability of this technique to grow will increase as sample types dictate. This will give the capability to develop rapid methods with high resolution of analytes. In HILIC with the power of MS detection, many analyte types can be resolved and we have highlighted a sample of 150 metabolomic species in a simple HILIC gradient
system.The diversity of the sample could potentially cause problems but the sharp peaks shapes provided by the homogenous silica surface of the Fortis HILIC aid in the resolution achieved.
1.7µm Fortis™ is a trademark of Fortis Technologies. ZIC® -HILIC is a registered trademark of Merck Sequant AB. All columns are original manufacturers own.
Available as 8 phase chemistries, choose from : 1.7µm C18 1.7µm H2o
1.7µm Diphenyl 1.7µm C8 1.7µm Cyano 1.7µm Amino 1.7µm HILIC
1.7µm HILIC DIOL
To request more information or a copy of the UHPLC brochure please contact:
info@fortis-technologies.com Tel: +44 (0) 151 336 2266
www.fortis-technologies.com
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