Volume 29 Number 6 2021 November


Awards


10 Microscopy Society of America Awards: 2021 Award Winners Miaofang Chi


Contents Biological Applications


16 Using MERSCOPE to Generate a Cell Atlas of the Mouse Brain that Includes Lowly Expressed Genes George Emanuel and Jiang He


20 Integrated Cryo-Correlative Microscopy for Targeted Structural Investigation In Situ Marit Smeets, Anna Bieber, Cristina Capitanio, Oda Schioetz, Thomas van der Heijden, Andries Effting, Éric Piel, Bassim Lazem, Philipp Erdmann, and Juergen Plitzko


Materials Applications


26 De-protonation of Nickel Hydroxide by 200 kV Electron Irradiation Graham J.C. Carpenter and Zbigniew S. Wronski


About the Cover


30 Small- and Wide-Angle X-Ray Scattering (SAXS/WAXS) with Temperature-Controlled Stages Makes Phase Identification


Faster than Ever Han Wu and Duncan Stacey


Microscopy 101


38 Out of the Blue and into the Black: Preparation, Mounting, and Image Rendering of Complex, Chorate Dinoflagellate Cysts for


Scanning Electron Microscopy Sandy M.S. McLachlan and Elaine C. Humphrey


Microscopy Education


Correlative FIB-milling fluorescence light microscopy and cryo-EM workflow.


See the article by Smeets et al. on page 20.


42 Remote Learning Facilitated by MyScope Explore Natalie P. Holmes, Matthew J. Griffith, Matthew G. Barr, Nicolas C. Nicolaidis, Vijay Bhatia, Michael Duncan, Ingrid McCarroll, Jenny Whiting, Paul C. Dastoor, and Julie M. Cairney


Microscopy Pioneers


50 Pioneers in Optics: Sir David Brewster (1781–1868) Cameron Varano


Departments


7 Editorial 8 Carmichael’s Concise Review


52 Industry News 54 Product News 56 Highlights from Microscopy and Microanalysis


58 Crossword Puzzle 60 NetNotes 71 Calendar of Meetings 73 Dear Abbe 74 Index of Advertisers


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