MicroscopyEducation
of microscopy and microanalysis core facilities across nine insti- tutions (University of Sydney, University of Queensland, Uni- versity of New South Wales, Australian National University, University of Western Australia, Flinders University, University of Adelaide, University of South Australia, Monash University). MyScope was developed by the participating universities to sup- port the face-to-face training offered within their facilities but has evolved into one of the best-known and heavily used train- ing tools for microscopy worldwide. Te MyScope SEM simulation is available in two differing
versions:
1. A simplified simulation of an SEM instrument that explains each of the different instrument controls and what their effect on an SEM image will be (for example, evacuate the chamber to remove air molecules that will interfere with the electron beam). Students are prompted to learn the influence of various controls through this environment, by loading simulated samples from the specimen library and choosing imaging conditions. Tis is “MyScope Explore,” which is aimed at a general and younger audience where they can both learn to use the SEM and also make discover- ies by exploring over 70 stored samples.
2. A realistic SEM simulation environment that mimics an actual state-of-the-art instrument. Te students can develop advanced skills through active learning activities designed to teach the impact of realistic setting changes on a sample under measurement, with a full range of controls including detector type and astigmatism correction. Tis is “MyScope,” a professional training environment.
Figure 4: Scanning electron micrographs depicting (a) blue plate-like scales (PLS) (Type I) and orange brush like-scales (BLS) (Type II) of a Maratus occasus (male) specimen (scale bar=10μm). (b) The internal structure of the plate-like scales of Maratus occasus that leads to the blue structural color (scale bar=2μm).
open-access nanostructural characterization capability and ser- vices, from pulsed-laser local electrode atom probe tomography and high-precision focused ion microprobes to high-resolution SEM and high-throughput cryo-transmission electron micros- copy. Te collaborative facility comprises a distributed network
Here we briefly detail the programming behind the
MyScope Explore graphical user interface (GUI) that makes the online interface possible. For each specimen type in the MyScope Explore library, the simulation is built using the HTML5 can- vas element. First, a series of scanning electron micrographs of the specimen are embedded, each at double the magnification of the previous item in the image string (Figure 5). Te canvas element is used to write three image filters: blur, contrast, and brightness. Te micrographs are then drawn onto the canvas, the canvas pixels are read back, and the three image filters run
Figure 5: A subset of the scanning electron micrographs of Maratus occasus embedded into MyScope Explore under the “Arthropods” category. (a) Zoom series for color 1 (blue plate-like scales, Type I). (b) Zoom series for color 2 (orange brush-like scales, Type II).
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