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Dutch Focus


What makes it possible for cacti to survive for years in a drought-ridden desert? How can so-called “resurrection plants” such as the Rose of Jericho survive months, even years, of extreme dehydration, only to be seemingly reborn when supplied with a small amount of water? Three researchers from the University of Leiden and the Delft University of Technology think they might know the answer


NADES could be missing ingredient in biological mysteries


Dr. Young Hae Choi and Prof. Robert Verpoorte of the University of Leiden, and Prof. Geert-Jan Witkamp of


the Delft


University of Technology, believe that their research into Natural Deep Eutectic Solvents (NADES) and their specific properties can help to shed light on a range of biological phenomena, and more importantly, solve long-running problems in the pharmaceutical and food industries. “It started about seven years ago when


we were doing a lot of metabolomics work,


trying to identify


compounds as possible present animal, or microbial cells,’’


as many in plant,


says Prof.


Verpoorte. ‘‘We noticed that they always contained quite a large amount of organic bases would


such as choline, more than you expect


in an appropriate


biosynthetic pathway.’’ Dr. Choi and Prof. Verpoorte discussed the implications of finding a significant


22


amount of choline in these processes, and concluded that it may point to the existence of a naturally occurring ionic liquid (IL). Synthetic ILs consist of an acid and a base in equimolar concentrations and are tailor made from different synthetic components for chemical and enzymatic


reactions. advantage is Their


volatile, these liquids last for ever. Experiments with


rise to liquids that being totally non- choline and some


common organic acids found in plants gave


that were able to


dissolve a wide range of poorly water soluble compounds commonly found in all kind of organisms. Further studies then led to the discovery of NADES, which are similar in properties to ILs but can be made by mixing neutral solid compounds like


sugars and sugar alcohols with


common amino acids, organic acids and bases as found in all organisms.


Once the presence of NADES had been


predicted and the physical proof of their existence had been provided, the team started to look at the biological significance of their findings. ‘‘Think about a flower standing in the


major


Sun for weeks. If the water in the flower is stored where all of the flower’s fragrance is kept, then after a day’s sunshine the fragrances will


be gone,’’ says Prof.


Verpoorte. ‘‘In reality, there is a NADES in the flower which remains after the water has gone, and helps the flower to retain its fragrance.’’ Dr. Choi and Prof. Verpoorte suggest that


the reason a flower is able to retain its fragrant properties in its nectar


is that


some of its component parts can combine to form a NADES with high water retaining capabilities. Essentially,


found in flowers that are solid at room temperature,


for example sugars and Insight Publishers | Projects the compounds


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