64 BIOTECHNOLOGY
Small-scale device for water-based protein solutions . The cGMP-validated self-
S
artorius Stedim Biotech (SSB), a leading supplier for the biopharmaceutical industry, has extended its product line of small-scale Sartocon Slice 50 crossflow devices with the Slice 50 ECO. This new crossflow filter has been specially designed for water-based protein solutions with viscosities below 3 cp. The crossflow cassette can typically be used in biotech applications, such as filtration of IgG, blood factors and peptides, especially involving membrane screening and small-volume process development. The reduced flow channel geometry
of Sartocon Slice 50 ECO lowers the recirculation pump requirements by as much as 50% versus a standard design. Only half of the pump energy is needed, considerably decreasing the sheer stress on all protein solutions. Like the standard Slice 50 cassette,
Sartocon Slice 50 ECO is offered as an encapsulated and ready-to-use crossflow module. Offered in a wide selection of molecular weight cut-offs, it has a filter
area of 50cm2
contained filtration unit eliminates the need for a compression holder and is available in a choice of two polymers:
membrane is extremely hydrophilic, making it non-protein-binding and virtually non- fouling. It is very easy to clean and delivers an exceptionally high product flux and product yield. “With our new product, we are offering
the industry a high-performance device for especially efficient filtration of low-volume aqueous protein solutions. Due to its features, such as low feed flow and low adsorption, Sartocon 50 ECO is a very cost-effective solution,” states Frank Meyeroltmanns, expert for crossflow filtration at SSB. Major benefits of the Sartocon Slice
The first polymer, a stable
polyethersulfone membrane (PESU) that is well established in the biopharmaceutical industry is ideal for a broad pH and temperature range. The second polymer, the Hydrosart membrane, is a stable, cross- linked regenerated ‘cellulosic’ polymer. It is also suitable for a broad pH range and can be cleaned and depyrogenated using up to 1N NaOH at elevated temperatures. This
50 cassette are its scalability and identical materials of construction. These properties ease transfer of process development results to the next scale-up level. In addition, the new filter device can be readily interfaced with SSB’s benchtop crossflow system Sartoflow Smart and with many other laboratory-scale filtration systems.
For more information visit
www.sartorius.com
New Treg isolation kit
he new MACSxpress Treg isolation kit, human, from Miltenyi Biotec facilitates both the fast and efficient isolation of regulatory T (Treg) cells from a maximum of 30ml of fresh, anticoagulated whole blood. The MACSxpress technique makes it possible to isolate Treg cell populations without using density gradient centrifugation and with only one
T
labelling step. Subsequently, the cells are immediately available for further analysis or cell cultivation. Treg cells play an important role in
regulating the immune response and peripheral tolerance mechanisms. In their role as the regulators of self-tolerance in the immune system, they prevent the onset of autoimmune diseases. However, they often suppress desired reactions, such as a
successful anti-tumour immune response, too. Their involvement in these immune mechanisms makes Treg cells particularly interesting for translational research. Miltenyi Biotec has developed
MACSxpress, a fast process for isolating cells from whole blood. The MACSxpress Treg isolation kit, human, enables the extremely efficient isolation of CD4+CD25+CD127dim/- Treg cells in only two steps. In the first step CD4- negative cells are removed using the immunomagnetic MACSxpress beads while erythrocytes are simultaneously sedimented. In the second step magnetic enrichment of CD25+ Treg cells is carried out using a MACS column. The marked Treg cells are held back in the column to be eluted and are then immediately available for cell cultivation or investigation.
For more information visit
www.miltenyibiotec.com
www.scientistlive.com
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