10 SPECIAL FOCUS: MICROPLATE READERS
dual emission detection can be used in any assay that measures two emission wavelengths or polarization vectors, including FP, FRET, HTRF and AlphaPlex.” T e advantage of miniaturisation allows rapid, inexpensive and eff ective measurement in 384, 1536 and 3456 well microplates. Pusterla comments that, “T e Pherastar FSX off ers a combination of features to support all major existing applications and future needs. T e reader easily performs applications such as protein- protein interactions, affi nity binding assays, compound and inhibitor screening as well as DNA, RNA and protein quantifi cation. Moreover, the microplate reader is suitable for enzyme activity, kinetic, cell based and reporter gene assays. T e Pherastar FSX especially provides excellent performance in all HTS applications, including NanoBRET, Alpha Technology and HTRF assays.”
Readers with research in mind Another well-known name in the world of microplate technologies is Molecular Devices, which
Fully modular microplate reading B
erthold Technologies has extended its TriStar2 multimodal microplate reader platform by
adding new functionalities to off er all main detection technologies in one easy- to-use, monochromator or fi lter-based
instrument. Developed for full modularity and equipped with the proprietary One-4-All optical system, the TriStar2 S series combines the user friendliness of a multimodal optical system with the sensitivity and performance of a dedicated optical device. Its maker says that it provides the fl exibility for today, tomorrow and beyond in a single system. Users can start with the reading technology they need to master their research today and upgrade when they need it. The TriStar2 S series is equipped with
both top and bottom reading technology to support a wide range of detection modes, including absorbance (UV/VIS), fl uorescence intensity (including FRET), fl uorescence polarization, luminescence (including BRET and BRET2), time
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resolved fl uorescence (TRF), and time resolved FRET (TR-FRET / HTRF). Up to three proprietary JET injectors can be installed to dispense multiple activators or detection reagents at any time with high accuracy and precision as well as excellent mixing performance. The option to inject in the measurement position off ers highest sensitivity for ultra-fast fl ash luminescence assays. A temperature- controlled microplate compartment ensures stable conditions whenever temperature-sensitive enzymes or cells are in use. The optional gas control unit enables adjustment of both O2 CO2
and gas levels, if required, for cell-based
applications. The upgradeable TriStar2 S multimode microplate reader off ers a fl exible all-in-one solution for any lab.
The SpectraMax i3x multi-mode microplate reader is Molecular Devices’ fl agship product
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