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BIOTECHNOLOGY 43


Fig. 1. The new sealing technology and advanced tip design of AcroPrep Advance filter plates result in faster, uniform filtration rates, improving well-to-well, plate-to-plate and lot-to-lot consistency


tradition of improving efficiencies and profitabilities of laboratory processes at all levels.”


Another new innovation from Pall is AcroPrep Advance filter plates. Tese provide high performance membranes in an optimised filter plate configuration for diagnostic, genomic and protein applications. New sealing technology and advanced tip design result in faster, uniform filtration rates, improving well-to-well, plate-to-plate and lot- to-lot consistency (Fig. 1).


Purifying and isolating biomolecules


Purification is key when it comes to isolating biomolecules. Here we look at the best of the latest filtration and purification technologies aimed at the biotech lab worker.


La purification est primordiale lorsqu’il s’agit d’isoler des biomolécules. Nous présentons ici les meilleures nouvelles technologies de filtration et de purification destinées au personnel de laboratoire spécialisé en biotechnologies..


Die Reinigung ist bei der Isolation von Biomolekülen entscheidend. Hier nehmen wir die besten der neuesten Filter- und Reinigungstechnologien für das Biotechlabor in Augenschein.


P


all Corporation’s new Acrodisc MS syringe filter is the first syringe


filter certified for low extractables in high performance liquid chromatography/mass spectrometry (LCMS) applications.


Designed specifically for LCMS, the Acrodisc MS syringe filter improves the accuracy of testing, enhances LCMS performance, and extends the longevity of testing instrumentation. Te filter’s low extractable levels minimise interference with the ionisation process and reduce the need for retesting. Tis lowers costs while reducing downtime due to unexpected contamination. To ensure high performance, product tubes are packaged individually so that filters not currently in use remain sealed and protected from external contamination.


Constructed with Pall’s new WWPTFE membrane in a high density polyethylene housing, the Acrodisc MS syringe filter can be used with both organic and aqueous solvents. It offers excellent chemical resistance, good particulate retention, and minimal protein adsorption. Tis protects instrument columns from particulate build-up and reduces the frequency and cost of replacement. It also enables more consistent LCMS testing performance.


“Pall’s Acrodisc MS syringe filter is the newest innovation in our comprehensive line of syringe filters and the first LCMS-certified syringe filter that is low in extractables,” said Larry O’Connell, senior vice president, Pall Global Lab Products. “Coupled with our other well-known filtration products, the Acrodisc MS filter will continue our


In addition, the company’s new Microsep Advance and Macrosep Advance ultrafiltration centrifugal devices simplify many common nucleic acid and protein handling procedures. Tis facilitates higher volumes of pure product and high recovery rates of up to 90 per cent. Ultrafiltration reduces the amount of handling that can cause damage to samples, leaving concentrated samples ready for direct incorporation into downstream applications at critical stages in the discovery process. Pall’s microfiltration centrifugal devices are used in separation and small-scale general filtration procedures, and can be combined with chromatography resins to create a fast, efficient method for purifying proteins.


Antibody polishing Polishing in antibody production is usually performed on quaternary ammonium (Q) membranes in flow-through mode, since the overall speed and productivity is much higher than on traditional anion-exchange columns. However, the binding capacity of Q ligands is reduced at higher conductivities, so concentrated feed streams must be diluted to adequately remove contaminants such as DNA, host cell proteins, viruses and endotoxins.


Te Sartobind STIC PA (primary www.scientistlive.com


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