chromatography • spectroscopy 25
one mixture,” said Harbaksh Sidhu, UPC2 programme director, Waters Division. “Te results were astounding. We saw repeatability, narrow, consistent peak widths, all in a gradient mode of operation with extremely low baseline noise. Te low system volume along with small particle size column is the result of a holistic design that unlocks the benefits of using compressed carbon dioxide that have never been realised before. As someone who’s been working with this technology for more than 18 years, I had never witnessed this level of performance before.”
Compressed carbon dioxide, the primary mobile phase for UPC2, offers numerous major advantages over liquid mobile phases or carrier gases that are used with LC and GC. For one, carbon dioxide alone, or combined with a co-solvent, is a low viscosity mobile phase that achieves higher diffusion rates and enhanced mass transfer than liquids used in HPLC. For another, when compared to GC, carbon dioxide
alone is a mobile phase that allows separations to occur at a much lower temperature.
Scientists can routinely separate compounds that are less amendable to analysis by LC and GC including molecules having a wide range of polarities.
Along with industry leading sub two-micron particle technology column chemistries, Waters ACQUITY UPC2 System gives scientists the ability to precisely vary mobile phase strength, pressure, and temperature. With this ability to fine-tune the resolving power and selectivity of the system, scientists can exercise better control over the retention of analytes for separating, detecting and quantifying structural analogues, isomers, and enantiomeric and diasteriomeric mixtures – all compounds that are often a challenge to separate by any other means.
Te company says that a key benefit of the system is the use of inexpensive
and non-toxic compressed carbon dioxide as a primary mobile phase that can take the place of toxic and volatile organic solvents that are extremely expensive to purchase and dispose.
Termo Fisher Scientific used Pittcon to launch the Termo Scientific TRACE 1300 Series GC, a compact, versatile gas chromatography system that is designed to increase productivity and lower cost in QA/QC and routine laboratories working on environmental, chemical and food safety applications. It features novel user-exchangeable instant connect injectors and detectors and an intuitive interface in instruments that deliver reliable results and maximum sensitivity.
Te TRACE 1300 Series GC comes in two models, the TRACE 1300 GC and TRACE 1310 GC. Te TRACE 1300 GC is ideal for budget-conscious labs carrying out routine analysis; its simplified interface requires limited user
interaction, so it can run 24/7 with limited staff oversight. Te TRACE 1310 GC, ideal for QA/ QC labs or labs focused on method development, features an icon-based touch screen interface for more precise user control.
Both systems benefit from robust design that is durable yet easy to use and maintain. Specific features include: user-exchangeable instant connect injectors and detectors that can be mounted in two minutes or less for quick changes if a different injection or detection technique is required; newly designed micro- volume detectors that ensure higher sensitivity and fast peak detection; optional injector back-flush option that eliminates heavy or undesired compounds to protect column and detectors, increase sensitivity and ease maintenance; and full control and easy programming with scalable Termo Scientific Dionex Chromeleon data system that streamlines path from samples to results offering enhanced operational simplicity.
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