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Flow Cytometry


Figure 3: The BD LSRFortessa™ X-20 can detect as many as 20 cell parameters simultaneously


24-tube racks, 96-, 384- and deep-well plates, and its smart sample-handling features such as temper- ature control and vortexing capabilities are designed to maintain cell viability and prevent clogging. Furthermore, the system can be integrat- ed into automated workflows and is ready to con- nect to laboratory information management sys- tems and scheduling software to support new or existing automation solutions.


ACEA Biosciences’ NovoCyte® Quanteon™ is a four-laser benchtop flow cytometer that can be configured for up to 25 fluorescent channels to support the screening of large-scale panels. The system incorporates the most sensitive silicon pho- tomultiplier detector technology on the market and


Figure 4


The Intellicyt® iQue Screener PLUS platform


offers a 7.2 log dynamic range, allowing both very dim and bright signals to be captured in the same analysis for the detection of rare events and low- abundance phenotypic markers. Combined with the NovoSampler Q™ autosampler, the system boasts rapid analysis speed and allows a 96-well plate to be processed in less than 20 minutes. Automated after-sampling rinse ensures minimal carryover between samples (<0.1%). The system is compatible with all of the most widely-used plate formats, from individual tube to 384-well plate, and can be integrated into automated workflows. The orbital shaker and barcode reader built in NovoSampler Q provide a complete walk-away solution when integrated with a laboratory automation system, while an Open Application Programme Interface allows for seamless operation with a scheduling software in a highly-automated format. Fully automated calibration can get NovoSampler Q up and running within five min- utes following installation (Figure 2).


BD Biosciences offers a range of flow cytometers for research and clinical diagnostics applications. The BD LSRFortessa™ X-20 cell analyser (Figure 3), for example, is a compact system built for high- performance multicolour analysis and can be con- figured with up to five lasers to facilitate the detec- tion of as many as 20 parameters simultaneously. Thanks to its fully-automated sample handling capabilities, the analyser can process a 96-well plate in under 15 minutes with less than 0.5% sample carryover. Its BD Accuri™ C6 Plus flow cytometer puts additional focus on usability and affordability, helping both novice and experienced users to benefit from flow cytometry. Incorporat- ing two lasers, two light scatter detectors and four


34


Drug Discovery World Spring 2019


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