Water monitoring
For this, they combined several different tracer techniques, namely the analysis of helium isotopes (to identify deep water enriched with mantle gases), vanadium (to identify deep water with its long and deep flow path), and microbial eDNA (Extracellular DNA) to identify extremophile microbes adapted to life at considerable depths/pressures. With these measurements, they could identify springs and shallow wells that received substantial deep groundwater inflow. Some of the wells most affected by deep groundwater were the larger wells used for drinking water.
Prof. Schilling adds: “As this is one of Japan’s most active seismic regions, we expect seasonal variations in deep groundwater upwelling and changes associated with seismic events such as earthquakes. For this purpose, we employ online flow cytometry alongside online dissolved (noble) gas measurements. “We continuously monitor the changes in the microbial composition in the drinking water well with BactoSense. We also conduct repeated spatial measurement campaigns where we sample different springs and wells for many hydrological tracers, including microbial eDNA. The eDNA samples are then analysed on
BactoSense via next- generation sequencing to match the flow cytometry fingerprints to metagenomic/ phylogenetic information.”
He continues: “So far, we have had a great experience with BactoSense. It is robust and transportable. With BactoSense’s continuous microbial monitoring, the team can detect deep groundwater pulses after earthquakes.
“We particularly like the ability to control the instrument remotely. It was set up in the drinking water well house within one day of work and has been running smoothly ever since. It is a good model for other volcanic
islands and coastal volcanic regions. Such systems are still seldom studied in hydrogeological terms. Our findings will help to develop monitoring techniques and protocols critical for sustainable and resilient
drinking water management in volcanic areas. The project shows how real-time microbial insight can protect critical water sources in complex geology worldwide.”
bNovate
www.bnovate.com
Instrumentation Monthly November 2025
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