Biomaterials
Optimal gelatin-based biomaterial
Rousselot Biomedical produces world-class, pharmaceutical-grade gelatins for ENLIGHT and other medical researchers and manufacturers that want to comply with the relevant FDA and EU quality and safety regulations.
ells in our body are attached or anchored to an extracellular matrix, therefore biomaterials derived from this matrix – like collagen and its denatured counterpart gelatin – can enhance the attachment and proliferation of cells promoting tissue repair. Gelatin’s natural biocompatibility and biodegradability make it an ideal biomaterial for in body applications. Since its discovery more than 20 years
C
ago, gelatin-methacryloyl (GelMA) has become the biomaterial of choice for a diverse range of biomedical applications, including wound dressing, bone regeneration, 3D bioprinting and tissue engineering. GelMA offers tunable mechanical properties that can be customised by varying the degree of modification and molecular weight, making it one of the most versatile hydrogels available for 3D cell culture.
Mimicking the natural cell environment
Cells originating from different tissues have different preferences for physiochemical
properties – for example, liver cells will prefer a softer environment to bone cells. The degree to which the cell culture conditions mimic the in vivo environment and recapitulate the natural behaviour and function of cells plays a critical role in enhancing cell survival, adhesion, and functional performance.
Biocompatibility can be enhanced by choosing a biomaterial that promotes mechanical stability at physiological temperatures and allows diffusion of essential oxygen and nutrients. Ideally, biomaterials should contain cell recognition motifs, such as integrin, that allow cells to physically adhere to the material and facilitate survival and growth. Biomaterial manufacturers can work with tissue engineers to formulate customised biomaterials that mimic the strength, elasticity and porosity of the natural cellular environment, whether it be skin, bones, or blood vessels. GeIMA scaffolding can then be customised and enriched with chemical properties and biological cues that are native to the tissue of interest.
Key characteristics to look for The first and most important characteristic for biomaterials for tissue engineering is that they are safe for use in the human body. Gelatin and GelMA hydrogels can contain soluble impurities, such as endotoxins. Endotoxins can initiate strong immune response that can lead to tissue inflammation, increased sensitivity to other allergens and the risk of fatal shock. Endotoxins can also have implications at the research stage, impacting cell growth and differentiation. Two other crucial factors to consider are consistency and scalability. Selecting a biomaterial with guaranteed batch-to- batch consistency at any scale can help avoid variability in your data. Choosing a biomaterial supplier that offers functional equivalence between research and GMP grade gelatins helps to minimise the need to revalidate R&D results ahead of non- clinical or clinical trials.
Creating a complex, fully functional tissue
An example of a novel application of gelatin biomaterials is the ENLIGHT project – a European collaboration developing a real 3D model of the pancreas to test new diabetes medications, using volumetric 3D bioprinting. The project uses X-Pure GelMA gelatin – an ultra-fast, visible- light induced, cross-linkable extracellular matrix (ECM)-like hydrogel – to mimic the pancreas structure.
Developments in tissue engineering, facilitated by innovative biomaterials, are enabling a new era of biomedical research and personalised medicines, reducing the need for animal testing and accelerating the delivery of life-changing treatments to patients. ●
Gelatin has become the biomaterial of choice for a diverse range of applications. 100
www.rousselot.com/biomedical Medical Device Developments /
www.nsmedicaldevices.com
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