UranyLess Applications All photos taken with TEM.


Parsley and Rosebush, Preparation of the sample using the following protocol: • Classic-Glutaraldehyde Fixation PFA, Osmium, Epon • Ultrafine Cup - Contrast UranyLess lead -citrate


Parsley Leaf. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Parsley Leaf. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Rosebush. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Culture Cells, Preparation of the sample using the following protocol: • Classic-Glutaraldehyde Fixation PFA, Osmium, Epon • Ultrafine Cup - Contrast UranyLess lead -citrate


Rosebush Root. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Rosebush Root. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Culture Cell. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Culture Cell. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Culture Cell. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Culture Cell. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Culture Cell. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


PLC Contrast Leica EM Stain Preparation of the sample using the following protocol: • Classic-Glutaraldehyde Fixation PFA, Osmium, Epon • Ultrafine Cup - contrast Uranyless Lead -Citrate


Spermatides Drosophile. Photo: Chantal Cazevieille Montpellier


Heart Headset. Photo: Chantal Cazevieille CRIC / IURC la'INSERM Montpellier (R & D - DeltaMicroscopies-France)


Chantal Cazevieille CRIC / IURC INSERM Montpellier tested aqueous UranyLess in the Leica brand grid contrast controller on different tissues, Drosophila heart atrium, retina, cochlea and ileum (Gut). The tissues were fixed according to the standard protocol 2.5% Glutaraldehyde in PHEM buffer, the post fixation in 0.5% osmium in 0.8% potassium ferrocyanide in RT for 2 hours. The sections are collected on single-hole or 200 mesh grids.


The treatment of the grids is UranyLess 7mn lead citrate followed 7 minutes.


We present here some images made by Hitachi transmission electron microscope with a digital camera AMT.


You will notice that the combined action of potassium ferrocyanide and UranyLess reveal a marked way the cyto-membranes in the ileum.


Drosophila. Photo: Chantal Cazevieille CRIC / IURC la'INSERM Montpellier


Drosophila. Photo: Chantal Cazevieille CRIC / IURC la'INSERM Montpellier


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Drosophila. Photo: Chantal Cazevieille CRIC / IURC la'INSERM Montpellier


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