UranyLess Applications All photos taken with TEM. Plant Tissue


Preparation of the sample using the following protocol:


• Glutaraldehyde Fixation Classic - Osmium - Included in Epon


• Contrast the UranyLess monitoring Lead Citrate


Plant Leaf. Photo: Jeannine Lherminier (INRA - Dijon)


Phage T6


Preparation of the sample using the following protocol: • Staggering Phage T6 on a G300-Cu grid Covered with a Carbon Formvar Film. Ionization 1 minute


Phage. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Cross-Sectional Bacteria


Preparation of the sample using the following protocol: • Fixing Classic Glutaraldehyde, Osmium, EPON


• Cutting Ultrafine, Double Contrast UranyLess and Lead Citrate.


Bacteria. Photo: Christine Longin (INRA Jouy en Josas).


Sacculina Crustaceans (Small Parasitic Crustacean) Preparation of the sample using the following protocol: • Classic Glutaraldehyde Fixation, Osmium, Epoxy Inclusion


• Fine Cups - Contrast to the Aqueous UranyLess to 60°C on a Hotplate without Lead Citrate Post Coloring


Sacculina (small parasitic crustacean) cuticle area. Photo: Djediat Chakib (Natural History Museum, Paris)


Liver Mouse and


Gerbil Sahara Preparation of the sample using the following protocol:


• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon


• Ultrafine Cup - Contrast UranyLess Lead - Citrate


Hepatocyte - Perinuclear Region. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Adrenal Gland


Gerbil Sahara Preparation of the sample using the following protocol:


• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon


• Ultrafine Cup - Contrast UranyLess lead -citrate


Adrenocortica. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Adrenocortica. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Bacteria. Photo: Christine Longin (INRA Jouy en Josas).


Intestine


Preparation of the sample using the following protocol:


• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon


• Ultrafine Cup - Contrast UranyLess Lead -Citrate


Intestine. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Bacteria. Photo: M2iSH team of Clermont Ferrand


Phage. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Phage. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Bacteria E. Coli Negative Staining for 2 Minutes UranyLess Bacteria Like E. Coli (Adherent and Invasive (ACSI) LF82) Which Have Pili and Flagella.


Phage. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


Plant Leaf. Photo: Jeannine Lherminier (INRA - Dijon)


Drosophila Larva Preparation of the sample using the following protocol:


• Classic-Glutaraldehyde Fixation PFA, Osmium, Epon


• Ultrafine Cup - Contrast UranyLess lead -citrate


Drosophila Larva. Photo: Nacer Benmeradi (R & D - DeltaMicroscopies-France)


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