UranyLess EM Stain A Substitute for Uranyl Acetate


EMS is proud to introduce UranyLess, a new contrast stain solution for TEM, for all of your negative staining applications. It is an amazing substitute for Uranyl Acetate with similar results.


After only a minute of contact, UranyLess’ fast-acting, non-radioactive lanthanide mix is finished staining your sections or deposits (see protocols below). If needed, lead citrate is recommended to increase the contrast.


UranyLess’s pH level is about 6,8 to 7. The 30ml airless bottle will stain approximately 1500 grids. The airless


bottle increases the shelf life, eliminates CO2 contamination, and produces less waste — the solution pumps out in perfect amounts without leaking or spilling. UranyLess is also available in a larger amount for use in automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.


UranyLess has been tested on many biological tissue (animal and plant): intestine, skeletal and cardiac muscle, liver, kidney, adrenal gland, nerve, cell culture, plant tissue, and also on negative staining of bacteriophage, bacteria, and polymers. UranyLess is ideal because of its ability to stain any kind of material and results are reproducible.


Lead Citrate 3%


UranyLess has a strong contrasting power, however, we recommend a Lead Citrate counterstain to enhance the contrast. You can follow the protocol of the UranyLess/Lead Citrate double contrast by watching the video. EMS recommends Lead Citrate 3%, ready to use. The special pump delivers the product without letting in air, thus preserving the solution and preventing CO2 dissolution. It is convenient to use because it performs in any position, even upside down. Comes in either a 30ml Airless bottle or a 30ml Airless Syringe. The Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100


30ml Airless Bottle


RT RT RT RT


Ordering Information 22409


UranyLess EM Stain*


22409-20 UranyLess EM Stain 22410


* in airless bottle ** Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100 References “Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids”


1. Delta Microscopies, 22, B route de saint Ybars, La côte blanche, 31190, Mauressac, France


2. Université Toulouse, CMEAB Faculté Medecine, 118 route Narbonne, 31062, Toulouse, France


Lead Citrate


Reynolds Stain 3%, Ready to use syringe


FREQUENTLY ASKED QUESTIONS...


What is UranyLess made from? UranyLess is a solution ready for use, a mix of lanthanides (rare-earths).


How is UranyLess sold? In an aqueous solution (water).


What is its shelf life? One year.


What are the storage conditions for UranyLess?


Store it at room temperature away from direct sunlight.


Does it need to be diluted? No, it is sold ready for use.


What is its pH? UranyLess pH is 6.8–7.


How to stain with UranyLess? Simply drop UranyLess on your grid, and wait a minute. Dry, then contrast with lead citrate according to Reynolds method.


Is it the same protocol for every kind


of tissue (animal, plant, marine)? Yes it is - a double stain of UranyLess plus Lead citrate.


Does it adjust to every kind of resin? Yes, it operates with every kind of resin (Epon, Araldite, Spurr).


Can it be used on negative staining? Yes, it can be used on negative staining.


Can it be used for bloc contrast? Some tests are in progress.


Is it efficient on marine material? Yes.


Is it adapted to a cryo use?


No, because it is prepared in water. However, we are currently developing many formulations of UranyLess, including ethyl UranyLess and acetone UranyLess, the latter being the best- adapted to cryogenic use.


How is UranyLess packaged? We sell UranyLess in an airless 30ml bottle and also in a brown 200ml bottle.


Can it be used with automated staining equipment?


Yes, the 200ml bottle is available for use with automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.


3. Microscopy Innovations LLC, 213 Air Park Rd, Suite 101, Marshfield, WI, 54449, USA


“C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle.” Nature Communications. Published 23 Apr 2015. Sandrine Floriot, all.


Lead Citrate 3%, Ready to use*


22410-01 Lead Citrate Reynolds Stain 3%, Ready to use syringe**


30 ml


200 ml 30 ml


30 ml


How does an airless bottle operate?


Its use is very simple; simply push on the head of the bottle to get a drop. When you release, the bottle back pump actuator lifts up. It prevents any air inlet in the bottle.


What is the advantage of an airless bottle?


It is a bottle in which air never enters. Some products, such as lead citrate, are atmospheric CO2 sensitive. Thanks to this system, those products have a longer shelf life. It also allows the product to be deposited drop by drop, quickly, cleanly and in any position.


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