Applications


Hydrophilization and cleaning of TEM grids carbon support films* for better sample spreading Improved adhesion and orientation of proteins, nucleic acids and antibodies TEM grid preparation for nanoparticle studies * Typically: Formvar®


, Lacey Carbon, Holey Carbon, Continuous Carbon, Quantifoil®


Touch screen control – rapid data input, simple operation New user interface has been extensively updated.


• Capacitive touch screen has improved sensitivity for ease of use


• User interface software has been completely revised, using a modern smartphone-style interface


• Comprehensive context-sensitive help screen


• USB interface allows easy software updates and backing up/copying of recipe files to USB stick


• Process log files can be exported via USB port in .csv format for analysis in Excel or similar. Log files include date, time and process parameters.


• 16GB flash memory can store over 1000 recipes • Dual-core ARM processor for a responsive display


Easy sample loading


Each of the twin chambers can accommodate two 25 x 75 mm glass microscope slides or TEM grid holders. Loading could not be easier using a drawer- style chamber door and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. The door and stages can be completely removed for convenience and to allow easy access for chamber cleaning.


Twin chambers prevent cross contamination


The GloQube Plus uses a single door with two independent vacuum chambers and adjustable sample stages. The in-air chamber is for simple glow discharge hydrophilic applications, while the in-vapor chamber is designed for hydrophobic (negative or positive) conversions, typically using reagents such as methanol and amylamine. By utilizing purge and flush cycles, we ensure contamination from the vapor chamber* does not affect the in-air chamber.


* For health and safety reasons when using the in- vapor chamber with chemicals the pump exhaust must be vented to a suitable external extraction system


Automatic Valve Stored Profiles


The automatic valve system allows greater control over the introduction of chemical vapor into the chamber. This results in reproducibility and repeatability of processing. The self-contained nature of the septum-sealed chemical vials and the delivery system ensures minimal user contact and a high level of operator safety.**


** Chemical preparation and disposal should be carried out in a suitable fume hood


Process control Selecting a New Profile


The GloQube Plus is an automated system, which has a recipe driven process control user interface for ease of use. Filtered inlets ensure that delicate samples, such as carbon coated TEM grids, are not contaminated with particles or dust. The GloQube Plus requires a single vacuum pump working in the 0.1 to 1 mbar range and has a typical pump downtime to operational vacuum of 60 seconds and a total cycle time in-air of usually less than two minutes*. A 750 mm flexible stainless vacuum hose is supplied with the GloQube Plus.


* Using a 30 second glow discharge process


GloQube Plus and Optional Pfeiffer DUO 6 Rotary Pump


A Typical Process Run Pumping Cycle


Easy Sample Loading


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