Rapid, reliable results... ®


GloQube Plus


Glow Discharge System for TEM Grids and Surface Modification


The GloQube® Plus is a cost-effective, compact and easy-to- Key Features


• Glow discharge treatment for electron microscopy sample preparation


• Automatic control of vapor and air introduction


• Flush and purge cycles of vapor chamber and gas line


• Two chambers for separate in-air and in- vapor processes without contamination*


• Fully automatic • Loaded with typical standard recipes


• Password protected user profiles and programmable user recipes


• Negative and positive discharge modes • Single door for easy sample loading • Adjustable three height sample stage • Intuitive touch screen control


• Safe vapor delivery using septum-sealed vials


• Automatic valving between chambers to prevent cross-contamination


• Fast and/or soft venting options • Extended warranty option * Only one chamber can be used at a time.


use glow discharge system, designed to fulfil the needs of laboratories with TEM.The primary application of the GloQube Plus is to modify the surface of TEM grids in a way that it meets requirements for successful imaging of a variety of macromolecules. Integrated into one system, the two chambers enable the user maximum flexibility to choose which sample preparation technique they want to use: glow discharge in-air or in-chemical vapor, without downtime for cleaning or the risk of contamination and loss of samples.


The in-chemical vapor glow discharge doesn't just help with retaining molecules on the TEM grids, but it also allows the user to control the orientation and conformation. With automatic vapor control, the system ensures accurate concentrations of chemical vapor in the plasma, producing reliable and reproducible results. Two chambers designed into one easy to use package provides a smaller footprint for the workflow space and no cross-contamination between the chambers.


Benefits • Short in-air cycle time


• Second chamber for separating in-air and in- vapor processes


• No cross-contamination between chambers due to post-process flush cycle


• Automatic vapor delivery ensures reliable and reproducible results


• Purge cycles reduce water vapor and oxygen concentrations, ensuring excellent yield of specifically orientated macromolecules


• Adjustable slow vent time to minimize sample disturbance


• Optional fast vent for rapid process times • Safe handling of reagent


• Three level adjustable height sample stage ensures repeatable results


Glow Discharge Process Surface State


Hydrophilic Hydrophilic Hydrophobic** GloQube Plus Start-up Screen Hydrophobic** Negative Methanol


Positive Positive


Surface Charge Atmosphere Negative


Air Air* Alkylamine Typical Applications


Hydrophilisation and cleaning of carbon coated TEM grids


Nucleic acid adhesion to carbon films


Controlled orientation and improved adhesion of negatively charged proteins, antibodies and nucleic acids


Controlled orientation and improved adhesion of positively charged protein molecules (e.g. ferritin, cytochrome c)


* Air followed by post-treatment with magnesium acetate by the user. ** Hydrophobic, as noted above, may represent a less hydrophilic sample of less than 90 degrees contact angle.


Vapor Delivery System Vapor Chamber Clean Chamber


Page 1  |  Page 2  |  Page 3  |  Page 4