C-flat™


Overview C-flat™


Holey Carbon Grids for Cryo-TEM The premier holey carbon grid for cryo-transmission electron microscopy


is an ultra-flat, holey


carbon-coated TEM support grid for transmission electron microscopy (TEM). Unlike competing holey carbon films, C-flat™


is manufactured without plastics, so it is clean upon arrival and the user has no residue to contend with.


The C-flat™ C-flat™


leads to better data sets. Made with patented technology, C-flat™


Advantage provides


an ultra-flat surface that results in better particle dispersion and more uniform ice thickness. Patterning is done using deep-UV projection lithography, ensuring the most accurate and consistent hole shapes and sizes down to submicron features. The precise methods by which C-flat™


is


manufactured eliminate artifacts such as excess carbon and edges around holes.


C-flat™ C-flat™


is affordable


is available in 25, 50, and 100 packs at a per-grid price less than competing products.


With 54 varieties, there is always a C-flat™ optimized for your needs.


Applications C-flat™


product


Hole Diameter Hole Spacing


TEM Grid Mesh


Insert of Carbon Film


TEM Grid Material


holey carbon grids provide the ideal specimen support to achieve high resolution data in cryo-TEM making them an ideal choice for single particle analysis, cryo electron tomography and automated TEM analysis.


Articles


Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Daniel Wrapp, Nianshuang Wang, Kizzmekia S. Corbett, Jory A. Goldsmith, Ching-Lin Hsieh, Olubukola Abiona, Barney S. Graham, Jason S. McLellan, 2020. Science, 13 Mar 2020: Vol. 367, Issue 6483, pp. 1260-1263, DOI: 10.1126/science.abb2507


An improved holey carbon film for cryo-electron microscopy. Quispe J, Damiano J, Mick SE, Nackashi DP, Fellmann D, Ajero TG, Carragher B, Potter CS, (2007). Microscopy and microanalysis, 13(5), 365-371.


Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box. Pierson J, Fernández JJ, Bos E, Amini S, Gnaegi H, Vos M, Bel B, Adolfsen F, Carrascosa JL, Peters PJ., J Struct Biol. 2010 Feb;169(2): 219-25. Epub 2009 Oct 12.


C-flat™ , CD-Flat™ , and Au-Flat™ , are trademarks of Protochips, Inc. All rights reserved.


2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles PDB ID: 3JCI Liu, Z., Guo, F., Wang, F., Li, T.C., Jiang, W. (2016) Structure 24: 319-328


Cryo-electron tomography (cryoET) and Single Particle Analysis (SPA): Numerous researchers have reported that the ultra-flat surface of C-flat™


leads to even ice


thickness and uniform particle distribution within the hole areas. This optimal particle distribution results in superior data being collected as compared with other holey support films. 2µm hole sizes are standard but custom hole sizes are available so C-flat™


can


accommodate the common magnifications used for quantitative TEM analysis.


provides a regular array of analysis sites compatible with automated data collection software such as Leginon. This compatibility, in combination with the more uniform ice thickness and particle distribution reported by numerous researchers, results in more high-quality target sites per grid.


Automated TEM: C-flat™


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