EMS BUFFERS & FIXATIVES EDITION I BUFFERS EDTA Buffer pH 8.5 (10x)


Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚ C. DO NOT FREEZE Intended Use: 1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10 X, pH 8.5 antigen retriever solution. Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2-8º C


Cat. No.


2-8˚C 2-8˚C


2-8˚C


64142-09 62142-10


Description


EDTA Buffer pH 8.5 EDTA Buffer pH 8.5


62142-10-1G EDTA Buffer pH 8.5


Michel’s Buffer Cat. No.


Description


RT RT RT RT


18041-05 18041-10


Michel’s Buffer Michel’s Buffer


18041-10-1G Michel’s Buffer 18041-10-5G Michel’s Buffer


Michel’s Transport Medium


Michel's Transport Medium allows for the shipping of specimens for further immunofluorescence work. It preserves morphology and antigenicity during transport. It is not a buffer or fixative. It is simply a vehicle to transport samples. The solution must be stored at refrigerated temperatures. Do not freeze. Samples may be stored up to 5 days in this medium at room temperature. z Comes in 250ml, 500ml, 1 Liter, Gallon, or 15ml fill in SecurTainer™


z Can be used with Michel's Buffer (washing solution) Cat. No.


Description


4–8˚ C 4–8˚ C 4–8˚ C 4–8˚ C 4–8˚ C


4–8˚ C


18042-05 18042-10 18042-15 18042-16 18042-17


Michel’s Transport Medium Michel’s Transport Medium Michel’s Transport Medium Michel’s Transport Medium


Michel’s Transport Medium, 15ml fill in SecurTainer™


18042-16-5G Michel’s Transport Medium Qty.


250 ml 500 ml 1 L


1 Gal. 50/cs


5 Gal. Qty. 100 ml


1000 ml 1 Gal.


Millonig’s Phosphate Buffer


Millonig (1964); Karlsson and Schultz (1965) Ingredients: Sodium Phosphate, monobasic (NaH2


Sodium Phosphate, dibasic (Na2 HPO4 •H2 Shipping Conditions: Ambient Temperature


The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.


Cat. No.


0–5˚ C 0–5˚ C


0–5˚ C 0–5˚ C


11582-05 11582-10


Description


Millonig’s Phosphate Buffer 0.2M Millonig’s Phosphate Buffer 0.2M


11582-10-1G Millonig’s Phosphate Buffer 0.2M 11582-10-5G Millonig’s Phosphate Buffer 0.2M


Qty.


500 ml 1 L


1 Gal. 5 Gal.


PO4 •H2 O); O); 0.5% Sodium Chloride (NaCl)


Qty.


500 ml 1 L


1 Gal. 5 Gal.


Electron Microscopy Sciences offers custom


and contract services, including Custom Chemical Manufacturing and Private Labeling. Please contact us for more information


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