EMS BUFFERS & FIXATIVES EDITION I BUFFERS Tris Buffer, pH 10.0 (10x)
This buffer is intended for heat-induced antigen retriever on formalin- fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
Cat. No. 4-8˚C
4-8˚C 4-8˚C
64142-11 64142-12
Description
Tris Buffer pH 10.0 Tris Buffer pH 10.0
64142-12-1G Tris Buffer pH 10.0 Qty. 100 ml
1000 ml 1 Gal.
Tris, (hydroxymethyl) aminomethane maleate Cat. No.
Description
0-5˚C 0-5˚C 0-5˚C 0-5˚C
RT RT RT RT
RT RT RT RT
RT RT RT RT
RT RT RT RT
Tris Maleate Buffer 11730 11740
11740-1L 11740-1G
Tris Buffer 11730-05 11730-06
Tris Maleate Buffer 0.2M pH range 6.4-8.0 Tris Maleate Buffer 0.2M pH range 6.4-8.0 Tris Maleate Buffer 0.2M pH range 6.4-8.0 Tris Maleate Buffer 0.2M pH range 6.4-8.0
Tris Buffer 0.2M pH 8.0 Tris Buffer 0.2M pH 8.0
11730-06-1L Tris Buffer 0.2M pH 8.0 11730-06-1G Tris Buffer 0.2M pH 8.0
Tris Buffered Saline 11730-08 11730-09
Tris HCL Buffer 11730-12 11730-15
Tris HCL Buffer 11730-20 11730-25
Tris Buffered Saline pH 7.4 Tris Buffered Saline pH 7.4
11730-09-1L Tris Buffered Saline pH 7.4 11730-09-1G Tris Buffered Saline pH 7.4
Tris HCL Buffer 1M pH range 6-8 Tris HCL Buffer 1M pH range 6-8
11730-15-1L Tris HCL Buffer 1M pH range 6-8 11730-15-1G Tris HCL Buffer 1M pH range 6-8
Tris HCL Buffer pH range 7.2-9.0 Tris HCL Buffer pH range 7.2-9.0
11730-25-1L Tris HCL Buffer pH range 7.2-9.0 11730-25-1G Tris HCL Buffer pH range 7.2-9.0
Qty.
250 ml 500 ml 1 L
1 Gal.
250 ml 500 ml 1 L
1 Gal.
250 ml 500 ml 1 L
1 Gal.
250 ml 500 ml 1 L
1 Gal.
250 ml 500 ml 1 L
1 Gal.
Your label, our label, custom volumes...
You can have your chemicals exactly the way you want them. Please contact us for more information.
P.O. Box 550 • 1560 Industry Rd Hatfield, PA 19440 Tel: (215) 412-8400 • Fax: (215) 412-8450
email:
info@emsdiasum.com
www.emsdiasum.com
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