EMS BUFFERS & FIXATIVES EDITION I BUFFERS Our Prepared Buffers are


All our ready-to-use buffers are: • Conveniently packaged.


• Prepared fresh for each order. • Economical and time-saving.


Desired pH should be specified when ordering.


Buffers are vehicles for fixatives and require certain properties: 1. Maintenance of constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.


2. The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.


RT RT


RT RT


RT RT


ready to use...


0-5˚C 0-5˚C


0-5˚C


Acetate Buffer Cat. No.


11480 11482


11482-1L


Acetate Buffer Acetate Buffer Acetate Buffer


Acetate Buffer Cat. No.


Description


11482-42 11482-50 11482-56


Acetate Buffer, 0.2M, pH 4.2


11482-42-1L Acetate Buffer, 0.2M, pH 4.2 Acetate Buffer, 0.2M, pH 5.0


11482-50-1L Acetate Buffer, 0.2M, pH 5.0 Acetate Buffer, 0.2M, pH 5.6


11482-56-1L Acetate Buffer, 0.2M, pH 5.6


Citrate Buffer, 0.1M Cat. No.


Citric Acid and Sodium Citrate 0.1M Description


0-5˚C 0-5˚C


0-5˚C


11490 11492


11492-1L


Citrate Buffer Citrate Buffer Citrate Buffer


Citrate Buffer, 0.2M Cat. No.


Citric Acid and Sodium Citrate 0.2M Description


0-5˚C 0-5˚C


11492-04 11492-06


Citrate Buffer, 0.2M, pH 4.0 Citrate Buffer, 0.2M, pH 6.0


Citrate Buffer, pH 6.0 (10x)


Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚C. DO NOT FREEZE Intended Use: 1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10 X, pH 6 antigen retriever solution. Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2–8ºC


Cat. No.


2-8˚C 2-8˚C


2-8˚C


64142-07 64142-08


Description


Citrate Buffer pH 6.0 Citrate Buffer pH 6.0


64142-08-1G Citrate Buffer pH 6.0 Qty. 100 ml


1000 ml 1 Gal.


Electron Microscopy Sciences offers custom


and contract services, including Custom Chemical Manufacturing and Private Labeling. Please contact us for more information


4


Acetic Acid and Sodium Acetate 0.2M Description


pH range 3.6-5.6 Qty.


225 ml 500 ml 1 L


Qty.


500 ml 1 L


500 ml 1 L


500 ml 1 L


pH range 4.6-6.2 Qty.


225 ml 500 ml 1 L


Qty. 1 L 1 L


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