and will be finalised with guidelines and recommendations by the end of 2020.
European partnership The new air-based method was developed by researchers in the following EU laboratories: National Food Institute in Den- mark, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘Giuseppe Caporale’ in Italy, Veterinary Research Insti- tute in the Czech Republic, National Veterinary Research Insti- tute in Poland, and the Norwegian Veterinary Institute in Oslo. The method yields test results in just two hours, using a type of mini-vacuum cleaner fitted with a special filter. This mini-vacuum cleaner collects the bacteria in the broiler house. The filter is then purified for total DNA and run in re- al-time PCR equipment. The PCR results indicate both the presence as well as the level of Campylobacter bacteria pres- ent in the dust of the chicken house. The test was developed collectively by the consortium, but the basic idea was initially developed and published by DTU back in 2009 (Olsen and others, Applied and Environmental Microbiology).
Only for closed sheds The researchers conducted comprehensive field trials over a two-year period which showed that the new method
quadruples the likelihood of detecting Campylobacter in a chicken flock. That is up to four times more chicken flocks showing signs of Campylobacter when compared with sock samples. According to the Danish Professor the new air test- ing system is useful in closed systems. He emphasizes that the system is only suitable for biosecured broiler houses where the inflow and outflow of air is regulated and the en- tire shed is a closed system with limited access. The capacity of this air testing system is based on the num- ber of air samplers (AirPort, Sartorius), but even with only one air sampler, a farm of up to 16 sheds can be tested in just one day. The way it works is that the air samples suck up to 750 litres of air during15 minutes of continuous testing. The air filter may be obtained for as little as € 1 per filter, while the air sampler, which is similar to a hand-held vacuum cleaner, costs approximately € 2000 (from Sartorius). The fil- ter samples are then treated for DNA purification (€ 3 per sample), which is tested in a PCR (€ 6). In total, only € 10 for a complete test result which covers an entire chicken house of up to 50,000 chickens for the entire six weeks of a produc- tion cycle. Compared to this the sock method involves selec- tive culturing, selective plates, and PCR or biochemical verifi- cation the cost of which which may even run to € 50 per sock sample.
▶ POULTRY WORLD | No. 6, 2020
Through better detection of Campylobacter, a better preven- tion strategy can be develloped.
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