NUTRITION ▶▶▶
Table 2 – Newcastle antibody titres in serum of pullets fed different diets. Negative control XTRACT® Nature Variation (%)
Mean titre 7’282
Coefficient of Variation (CV%) 71% Titre range of reference1
7’000 – 13’000 1: Both groups were within the “titre range of reference” provided by the laboratory
11’712 55%
+ 60.8% -16%
demonstrated when they are used in combination. The first, curcuminoids (contained in turmeric oleoresin) has been used for centuries in Indian and Chinese medicine. It im- proves and speeds up the antigens recognition by the host and therefore enhances antibodies production. The second, capsaicinoids (contained in chilli pepper oleoresin) is known to exert a strong anti-inflammatory activity. This ingredient has repeatedly demonstrated its ability to limit both local and global inflammatory response (Lee et al., 2010). Being irritants and difficult to handle, these two ingredients have to be micro-encapsulated with an adequate matrix in order to create an additive that can be used in animal feeds. The interest of the addition of such additives made of both tur- meric and chilli pepper oleoresins (XTRACT Nature) in feed and as a supplement to a vaccination programme, was first evalu- ated by the USDA (United State Department of Agriculture) Animal Parasitic Diseases Laboratory in 2011 for broilers. Re- sults demonstrated a boosting effect of the cocci-vaccination and an increase in the pool of antibodies produced.
Improve vaccine response In a recent trial performed in South East Asia, the efficacy of XTRACT Nature has been tested in Newcastle disease vacci- nated pullets. This trial lasted 12 weeks and the objective was to confirm the efficiency of the additive. Two buildings were selected in a commercial farm with 5,000 pullets/building. Pullets were from Dekalb white genetic. From day 9 onward, they received the same commercial diet, XTRACT Nature at 100g/ton being the only difference between the two groups. According to the farm vaccination programme, all pullets were vaccinated against Newcastle Disease (ND) at 19 days of age. At day 32, three pullets per treatment were necropsied and at day 46 (27 days post vaccination), the blood of 20
Figure 1 - Trial timeframe. Necropsy results Day 0
Day 9 Arrival Test diets
Newcastle Vaccine
Necropsy 26 Day 19
pullets per treatment was sampled and analysed by ELISA for Newcastle antibody titres. The trial timeframe is illustrated in the figure 1 below. At the necropsy (day 32), signs of inflammation were identified in the trachea of the pullet from the control group. This inflam- mation is most probably due to a response of the innate im- mune system to the vaccine. Pullet receiving the product re- vealed lower inflammation status.
Day 32 27 days post-vaccination
Blood samples Newcastle titers analysis
▶ POULTRY WORLD | No. 6, 2019
End of the trial
Day 46 Week 12
Titre response following vaccination Serum ELISA analysis for Newcastle antibody titres revealed that pullets supplemented with the standardised phyto-mol- ecule additive had a marked increase of Newcastle titres (11,712 vs. 7,282) together with a lower coefficient of varia- tion (55% vs. 71%) compared to the control. In other words, pullets receiving the product had higher protective levels and a more uniform flock protection against Newcastle disease (see figures 2 and 3 and table 2). Last but not least, pullet body weight was measured at the end of the trial (12 weeks of age). Pullets having taken XTRACT Nature had higher body weight (945.4 g/pullet) than pullets of the negative control (914.4 g/pullet). When fed with the product, pullets achieved 98.8% of their growth
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