sections where pH is considerably lower than in subsequent sections. Enzymes with a superior pH profile will thus hydro- lyse phytate in the upper part of the digestive tract as quickly as possible to effectively reduce the antinutritional effect of phytate. Pelleting, a process widely used in feed production in which high temperature and pressure are used, also im- pairs the stability of added feed enzymes and represents a major concern for feed manufacturers. However, the phytase market is continuing to develop and test novel enzymes and formulations in an effort to find new, more suitable enzymes that can cope with the challenges faced during feed production and are tailored for optimal swine nutrition.
Better does not have to be enemy of good The above-mentioned high bioefficacious phytase was fur- ther improved, resulting in the new Axtra PHY GOLD. Its per- formance in vitro followed by in vivo trials validated how it excels at replacing phosphate supplementation in animal feeds and also, for example, improving growth and digestive performance in pigs. Phytate in vitro trials were run with five different phytases and these assessed enzymatic perfor- mance over a range of pH, phosphorus release and harsh pel- leting conditions. Widening the enzyme’s pH ranges ensures better perfor- mance in very acidic environments such as the upper diges- tive tract, thus ensuring faster phosphorus release into the di- gestive system. The recently developed phytase had a superior pH profile compared to competitors (Figure 1), as well as higher thermostability when exposed to high pellet- ing temperatures, which is vital to avoid a reduction in the amount of available enzyme available in feed formulation. Superior phosphorus release results were also recorded when compared to four phytases currently available on the market. In vivo studies in sows and piglets fed a corn-based diet showed that the new phytase enzyme can completely re- place all inorganic phosphorus supplementation in piglet di- ets while maintaining FCR and growth performance levels, compared to the positive control group. The piglets’ final body weight was also higher with the new enzyme, and this
was validated in a trial in which the phytases were supple- mented at 250, 500 or 1,000 FTU/kg (Figure 2). Phosphorus di- gestibility is a key indicator of how successful a phytase is in making phosphorus availabile to the animal. In a trial in which sows were fed diets supplemented with phytase at 500 FTU/kg, the new phytase significantly improved total tract phosphorus digestibility by 9.6% compared to Buttiaux- ella phytase. The recently developed phytase thus has a high phosphorus release rate, which makes phosphorus available for absorption during digestion.
A new phytase with a great deal of promise The new product had superior thermostability, enabled the total removal of inorganic phosphate from plant-based diets, improved swine performance and resulted in a better envi- ronmental footprint. It also proved to be especially useful for pre-mixers that have no control over their final customer’s pelleting conditions. Because even great enzyme performers can still be improved, enzyme tailoring remains a field worth continued exploration.
References available on request.
Figure 2 - Effect of increasing phytase doses from 0 to 1,000 FTU/kg on the final body weight of piglets.
Final Body Weight (day 28)
28 29
24 25 26 27
0 250 a Axtra PHY GOLD Buttiauxella phytase PC ab abc abc bc
NC3 NC2 NC1
500 Phytase dose (FTU/kg feed) ▶ ALL ABOUT FEED | Volume 28, No. 4, 2020 19 750 1000
The new phytase enzyme can completely replace all inorganic phos- phorus supple- mentation in piglet diets while maintain- ing FCR and growth performance levels.
Body Weight (kg/pig)
PHOTO: VAN ASSENDELFT FOTOGRAFIE
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