MICROSCOPY & IMAGING
Dr Kamal Nahas (graduate student at
University of Cambridge) at B24 working on data showing the progress of herpes virus particles in the interior of human cells
mechanism that it employs to travel and propagate within human cells. Te B24 team are experts in the
preparation, handling and transfer of cryopreserved samples. Tey work closely with the user community to help researchers get the best results possible and plan experiments that make the best use of the available technology.
INSTRUMENTATION AND APPLICATIONS Te beamline delivers SXT using radiation from a bending magnet at the synchrotron, which is then reflected and conditioned via a number of specially designed mirrors and ultimately it is delivered as a highly focused 500 eV beam to an X-ray microscope (UltraXRM- S220c, Zeiss) at 500eV. Samples are mounted on round 3mm wafers and kept at cryogenic temperatures at all times. Data collection can be driven in person or remotely and raw imaging data enters an automated pipeline for reconstruction and processing.
For SIM experiments at cryogenic temperatures, the cryoSIM microscope was developed through the collaborative work of the B24 team and Micron, the microscopy facility at the Biochemistry department of the University of Oxford. Bringing together the resident expertise on cryo-handling, sample optimisation and data collection and the wealth of knowledge from microscopy developers in academia resulted in the construction of a user-friendly and accessible super resolution fluorescence module that perfectly complements the SXT facility. It is always used before exposure to X-rays depletes fluorophore capabilities and provides 3D data on chemical localisation based on the fluorescence of the trackers used. Tis data can show details even below 200nm resolution and the reconstruction process delivers high-contrast, low- background imaging beyond the diffraction limit of the light used.
Te technology at B24 has been used so far to study eukaryotic cells, algae, archaea, pathogens (parasites, viruses
Representative images of two mammalian cells showing the distribution of filamentous actin in their cytoskeleton
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