CHROMATOGRAPHY
Landon Wiest introduces a new approach for analysing acrylamide in food AANALYSING A
crylamide is a hazardous chemical that forms in food when sugars and the amino acid asparagine react during high-heat cooking
processes such as frying, roasting, grilling or baking. Te detection of acrylamide in food was first reported in 2002 by the Swedish National Food Administration, and, since then, global efforts have been growing to better understand its origin in food and feed, the magnitude of contamination in various foodstuffs, and the potential health effects related to dietary exposure. To safeguard public health, more efficient methods for analysing acrylamide in foods and beverages are needed. Here, we compare typical approaches to an improved process that yields faster analysis times and longer periods of sample throughput without system suitability failures.
CURRENT ANALYTICAL APPROACH Europe has formalised a method for acrylamide analysis in food – EN 16618:2015 – and it calls for the use of LC guard and analytical columns that contain porous graphitised carbon (PGC), in part because of the difficulty of analysing acrylamide using more traditional reversed-phase (RP) retention mechanisms. PGC columns are now commonly used because they can retain acrylamide sufficiently to separate it from coextracted matrix components. Te PGC column is also able to operate in 100% aqueous mobile phase, a condition under which many RP columns would undergo a phenomenon known as “dewetting,” which results in retention time loss and requires that the column be regenerated by a time-consuming flush with 100% organic mobile phase. Following method EN 16618:2015, eight minutes are required to analyse acrylamide in food. To meet system
www.scientistlive.com 25 CRYLAMIDE
FIG. 1. Labs can increase sample throughput using an Allure Acrylamide column because much less equilibration time is needed compared to a PGC column. Peaks: (1) acrylamide-d3, (2) acrylamide. For sample preparation details and instrument conditions, visit
www.restek.com and enter LC_FS0532 in the search
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