MICROPLATE READERS
HIGH-PERFORMANCE MICROPLATES INTRODUCED T
he Sero Krystal PDL range of coated microplates from Porvair Sciences aim to set a new benchmark for reproducible cell culture even with challenging cell types. Many scientists would prefer to grow cells in a serum-free environment to more accurately mimic physiological conditions. However, this often presents challenges as cell lines often struggle to attach to traditional tissue culture plastics in the absence of serum. Manufactured from ultra-pure polystyrene in a clean room environment, Sero Krystal Poly-D-Lysine (PDL) coated microplates were developed to overcome these challenges and promote superior cell attachment, growth and proliferation for a wide range of cell types in serum-free media. Poly-D-Lysine is a synthetic (animal-free)
extracellular matrix used in cell culture applications to promote cell adhesion. Available in 96- and 384-well formats these new microplates are uniformly coated with a 70 to 150 kDa PDL polymer using proprietary methods. The coating creates a uniform net positive charge on the surface of the plate wells. This positive surface
charge improves electrostatic interactions between the coated surface and the negative charges of the cell membrane resulting in improved cell attachment. Sero Krystal microplates are ideal for the culture of transfected cell lines, primary neurons, glial cells and fibroblasts, such as the adherent mouse fibroblast cell line L929 and other common adherent cell lines including HEK-293, BHK-21, PC12 and, NSC-34. Available in clear, black and frame
versions, the microplates incorporate an optically clear base. The consistent flatness
of the base gives superior light transmission for high resolution imaging and a flat optical plane for growing cells. Supplied sterile and ready-to-use, Sero Krystal PDL coated microplates are the first part of the new Sero range of cell culture plates to launch.
For more information visit
www.porvairsciences.com
NEW MULTI-FUNCTIONAL CONFOCAL IMAGING READER T
he recently launched Agilent BioTek Cytation C10 confocal imaging reader is a versatile and multi-functional automated system that is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and
quantitative data to support cell-based research.
Building upon the legacy of the popular
Cytation cell imaging multimode readers, the compact new system combines automated digital confocal and widefield microscopy with conventional multi-mode microplate reading in a novel design. Spinning disc confocal capabilities offer excellent image resolution and optical sectioning for a variety of sample types including three-dimensional (3D) cell cultures and thick tissue sections.
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High-quality components, such as a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser- based illumination support excellent publication-ready images. With its modular design the Cytation C10 may be upgraded at any time as laboratory needs evolve. Additional imaging modes include widefield fluorescence, brightfield, and phase contrast optics. Assay versatility is further expanded with variable bandwidth monochromator- based optics for detection-based applications, and environmental controls for long-term live cell imaging analysis. Agilent BioTek Gen5 software powers
the new solution offering a 3D viewer and automated ROI functionality, among other powerful processing and analysis features.
For more information visit
www.agilent.com
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