MICROPLATE READERS
LAMP products are detected by absorbance or fl uorescence measurement, ELISAs for the quantifi cation of antibodies and viral antigens or cytokines in serological samples rely on a colorimetric, fl uorescent, or luminescent output. As all these detection methods can be incorporated in a single device, microplate readers are a viable option for performing large quantities of diff erent diagnostic tests due to their high throughput and sensitivity.
SCREENING FOR ANTI-VIRALS Anti-viral screenings are used to discover drug-based treatment options for viral infections. Once druggable targets are identifi ed, assays for the detection of their activity need to be scaled up to effi ciently screen for anti-viral molecules. For this purpose, labelled substrates are employed to allow plate reader-based detection of enzymatic assays such as neuraminidase or RNA-dependent RNA Polymerase activity and inhibition. FRET, BRET, TR-FRET, fl uorescence polarisation and AlphaScreen are extremely useful for the analysis of virus-host protein interactions in high- throughput and of utmost importance for the identifi cation of inhibitors of these interactions. Furthermore, the detection of viral replication in host cells can be used as readout to assess the potency of
FIG. 1 Kinetic measurement of the SARS- CoV-2 LAMP assay over 60 min. Data is displayed as ∆OD (415 nm-560 nm). The optional measurement window is marked in orange
anti-virals (Fig. 2). Irrespective of the method, microplate readers with highest sensitivity and speed are essential for high-throughput screening approaches, delivering robust and reproducible results for thousands of samples. To achieve even higher throughput, many microplate readers are robot compatible and can easily be integrated into automated systems. T e BMG Labtech Clariostar Plus
plate reader with LVF Monochromators,
temperature and atmospheric control capabilities is an ideal tool for virology research. For high-throughput screening applications, the Pherastar FSX provides excellent sensitivity and detection times.
Martin Mangold is with BMG Labtech.
www.bmglabtech.com
FIG. 2 (A) Anti-viral screening (B) Comparison of images and well- scans showing viral replication in infected cells either non- treated or treated with diff erent drugs. Images were taken with an imaging device, well- scans were performed on a microplate reader
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