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November/December 2009 Analysis of Total Parental Nutrition Solutions


Metrohm has developed a method for the analysis of Total Parental Nutrition (TPN) solutions that are often administered to particularly vulnerable patients for example sick or newborn babies. TPN is a method of feeding that bypasses the gastrointestinal tract where the fluids are given into a vein to provide most of the necessary nutrients that the body needs.


Typical electrolytes within a TPN solution include acetate, chloride, sodium, potassium, calcium and magnesium. Metrohm has developed methods for the analysis of both anion and cation analytes within TPN solutions using their Intelligent IC instruments. Total Parental Nutrition solutions represent a challenging sample matrix and deliver a mixture of fluid, electrolytes, calories, amino


Analysis of Antioxidants in Red Wine


Thermo Fisher Scientific announce a new application note entitled “Direct Analysis of Red Wine Using Ultra-Fast Chromatography.” The application note explains the unique capabilities of ultra high pressure liquid chromatography (U-HPLC) coupled to a Thermo Scientific Orbitrap mass spectrometer for use in the identification and quantitation


of antioxidant constituents in red wine. The LTQ Orbitrap XL™ hybrid mass spectrometer combined with Accela™ U-HPLC delivers fast analysis times and accurate mass measurements, confirming the identity and structure of compounds in these complex mixtures. The application note is available for download at www.thermo.com/redwine


There is a growing interest among nutrition and health researchers in advanced liquid chromatography and mass spectrometry techniques. Red wine is a highly complex mixture and a rich source of beneficial antioxidants. It contains natural compounds that may, if consumed regularly and in moderation, have a positive impact on human health.


In complex matrices, however, as is the case with red wine, identification and quantitation of natural compounds is challenging.


The application note demonstrates the potential of a direct analysis of red wine using the high resolving power of both the Accela and the LTQ Orbitrap XL. Researchers identified phenolic compounds responsible for most health benefits associated with red wine, such as quercetin, and they observed variable content across two different harvest years. Comparative analyses were performed using Thermo Scientific SIEVE 1.2 software. The features were filtered for statistical significance, with significant change defined as a minimum of two-fold concentration difference between the two harvest years. The results presented in the application note show statistically significant changes for individual compounds detected across two harvest years. The concentration differences for some compounds, particularly quercetin, were considerable.


The LTQ Orbitrap XL delivers extremely high mass accuracy and resolution and is capable of multiple levels of fragmentation. The Accela U-HPLC affords fast analysis time while maintaining very high chromatographic resolution, which is important for complex matrices. The combination of the LTQ Orbitrap XL with Accela U-HPLC provides a robust and confident means of profiling complex mixtures as well as successful identification and advanced structural characterization of detected compounds without prior sample preparation.


acids, vitamins, minerals and often fats into an infant's vein.


Intelligent IC can be used to assay both the anion and cation constituents usually with analysis times of less than 20 minutes and the sample preparation is often no more complicated than a simple dilution. The intuitive nature of the MagIC Net controlling software means that the instrument can be run and a result obtained by a technician literally with a couple of mouse clicks.


The electrolytes play a number of important roles within the body including regulation of hydration, blood pH and nerve & muscle function. It is vitally important that the constituents are carefully monitored as an ion imbalance can cause serious illness and in the event of a serious overdose can cause liver problems and has unfortunately resulted in infant death in a number of cases.


Monitoring functionality and control charts within the software mean that the TPN assays can easily be profiled over a period of time and should values be outside of a strictly defined tolerance than the user is immediately notified through the software so that remedial measures may be instigated.


For further information please contact enquiry@metrohm.co.uk


Advertise in our next issue... contact us on +44 (0)1727 855574, chromatographytoday@ilmdesigns.co.uk or visit www.chromatographytoday.com ...we look forward to hearing from you


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