Microscopy101
Making Your Own Hair Probe: A Letter to the Editor
Gayle M. Callis GCallis Histology Service , LLC , 1442 Ash Drive , Bozeman , MT 59715
gayle.callis@
bresnan.net
To the Editor:
I read with great interest the Microscopy 101 article, “Making Your Own Hair Probe: A Simple Guide” [Bob Chiovetti, Microscopy Today 22(6) (2014) 46]. We made hair probes for TEM in the early 1960s using human eye lashes mounted with pink dental wax onto shortened applicator sticks. A word of caution was given about using eyelashes coated with mascara. We were told mascara can flake off and cause contaminates, that is, “floaters,” which could end up on sections and grids. Photos of a mascara-coated eyelash and an uncoated eyelash have been donated by Phil Oshel ( Figure 1 ). My compliments to Bob Chiovetti for providing excellent details on how to make hair probes. This brought back fond memories of working in an EM laboratory so many years ago.
Respectfully yours,
Gayle M. Callis HTL/HT/MT (ASCP)
Figure 1 : Eyelashes from the same person. Left, with mascara, and right, a clean eyelash.
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doi: 10.1017/S1551929515000802
www.microscopy-today.com • 2015 September
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