Capsule-Based Processing and Handling
evaluated 16 diff erent tissue fi xation, embedding, and staining protocols [ 16 ].
Conclusion T e mPrep capsule processing method can improve the
ease, effi ciency, and reproducibility of TEM and SEM sample preparation protocols. Capsule processing can provide advantages that include identical and simultaneous preparation to increase reproducibility, to provide ten-fold or greater reduction of reagent consumption, and to aff ord a similar reduction in technician eff ort. T is report shows that effi cient processing, producing quality results, was achieved by multiple labs in three diff erent institutions.
Acknowledgments T e authors acknowledge the assistance of Patricia Kierski and Jonathon McAnulty (University of Wisconsin School of Veterinary Medicine) for providing fresh pig tissues, and Janice Pennington (Department of Botany, University of Wisconsin) for helpful advice. We also acknowledge the use of instrumentation supported by the University of Wisconsin Advanced Materials Industrial Consortium (DMR-1121288) and the Nanoscale Science and Engineering Center (DMR-0832760).
References [1] GE Palade , J Exptl Med 95 ( 1952 ) 285 – 98 . [2] KR Porter and J Blum , Anat Rec 117 ( 1953 ) 685 – 710 .
[3] A Claude and EF Fullam , J Exp Med 83 ( 1946 ) 499 – 504 . [4] K Porter et al ., J Exp Med 81 ( 1945 ) 223 – 46 . [5] GE Palade , Science 189 ( 1975 ) 347 – 58 . [6] WE Gilson and RE Gilson , Adjustable Pipette. US Patent 3827305A, fi led Oct 24, 1972, published Aug 6, 1974.
[7] NM Cooke and PH Hall , US Patent 3356462A, fi led Aug 9, 1966, published Dec 5, 1967.
[8] R Manns . Microplate History , 2nd Ed., presented at MipTec-ICAR’99, May 17–21, 1999, Montreux, Switzerland.
[9] T Astle , Journal of the Association for Laboratory Automation 5 ( 2000 ) 30 –3 1 .
[10] JJ Bozzola and LD Russell , Electron Microscopy , 2nd Ed. , Jones and Bartlett , Boston , 1999 , p. 19 .
[11] ES Reynolds , J Cell Biol 17 ( 1 ) (1963 ) 208 – 12 . [12] JJ Bozzola and LD Russell , Electron Microscopy , 2nd Ed ., Jones and Bartlett , Boston , 1999 , p. 126 – 29 .
[13] MJ Karnovsky , J Cell Biol 27 ( 1965 ) 137A . [14] KD Wendt et al ., J Microsc 214 ( Pt 1 ) (2004 ) 80 – 88 . [15] Microscopy Innovations, LLC, “Immunolabeling with mPrep Capsules - Protocol, and Template for Laying Out Reagents in 96-Well Plates,” in “Application Notes.”
http://microscopyinnovations.com/Tech-info.php (accessed July 3, 2015).
[16] M McClain , Microsc Microanal 20 ( Suppl 3 ) (2014 ) 1288 – 89 .
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www.microscopy-today.com • 2015 September
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