bringing correlative light and electron microscopy forward


CryoCapsule ®


Designed to accelerate, facilitate and standardize sample manipulations throughout the CLEM workflow.


EMS has it! CryoCapsule


®


Sample Preparation Device for Correlative Light to Electron Microscopy


The CryoCapsule® is


a new tool in the field of High Pressure Freezing (HPF) and correlative light and electron microscopy (CLEM). Comparable to a small petri dish, it is composed of a landmarked sapphire disc and a gold spacer ring (50µm thick) maintained together by a plastic ring[1]


.


The specimens are encapsulated between the support sapphire disc (carbon landmarked) and a covering sapphire disc.


The CryoCapsule® is loaded into a specific


adaptor and live cell imaging can then be done directly on the specimen prior to HPF.


Post-HPF, the specimen is processed for freeze substitution[2] temperature sectioning.


and room


CryoCapCell has also developed a set of tools to manipulate the CryoCapsule® [2] most scientific environments.


in


CONTACT US FOR MORE INFORMATION...


References 1.


2.


Heiligenstein X, Heiligenstein J, Delevoye C, Hurbain I, Bardin S, Paul-Gilloteaux


P, Sengmanivong L, Régnier G, Salamero J, Antony C, Raposo G. The CryoCapsule: Simplifying Correlative Light to Electron Microscopy. Traffic [Internet] 2014 [cited 2014 May 14];15:700-16.


Heiligenstein X, Hurbain I, Delevoye C, Salamero J, Antony C, Raposo G. Step by step manipulation of the CryoCapsule with HPM high pressure freezers. Methods Cell Biol [Internet] 2014 [cited 2014 Nov 27];124:259-74.


P.O. Box 550 • 1560 Industry Rd. Hatfield, Pa 19440 Tel: (215) 412-8400 Fax: (215) 412-8450 email: sgkcck@aol.com or stacie@ems-secure.com


www.emsdiasum.com


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