Experts in Vacuum for Science


Table 1 – MVS system performance specifications for Range E Sample Solution in 96-well Verification Plates


Volume range


0.999 µL–0.2000 µL 0.1999 µL–0.1000 µL ≤0.0999 µL


Accuracy (%) 2.0 3.0


N/A


CV (%) 0.4


0.4 N/A


Table 2 – MVS system performance specifications for Range E Sample Solution in 384-well Verification Plates


Volume range


0.299 µL–0.0500 µL 0.0499 µL–0.0200 µL 0.0199 µL–0.0100 µL ≤0.0099 µL


Accuracy (%) 2.5 3.5 5.5


N/A


CV (%) 0.8


0.8 0.9


N/A


Tables 1 and 2 demonstrate the MVS per- formance capabilities for both aqueous and DMSO Range E Sample Solutions over the volume range of 100–999 nL in 96-well plates, and the expanded volume range of 10–300 nL in 384-well plates.


Conclusion The data herein demonstrate that 100% DMSO


Range E Sample Solution adds a significant new capability to the MVS for measuring liquid handling instrument performance. In turn, this should answer the need for those scientists working in the drug discovery arena for a fast and reliable method to verify that their liquid handling instruments are accurately and precisely dispensing both water- and DMSO-based solutions.


The development of this DMSO solution has also opened the door for higher-volume-range DMSO-based MVS Sample Solutions.


References 1. Albert, K.J.; Bradshaw, J.T. Importance of


integrating a volume verification method for liquid handlers: applications in learn-


ing performance behavior. J. Assoc. for Lab. Automation, June 2007, 12(3), 172–80.


2. Hentz, N. Impact of Liquid Handling Vari- ability on In Vitro Biochemical Assay Per- formance: A Look at Protein Binding and Enzyme Assay Classes. Presentation given at SLAS 2013, Orlando, FL; http://goo.gl/ zNmG9.


3. Albert, K.J. Optimize Volume Transfer Methods to Avoid Reagent Concentration Errors of >50%. Presentation given at SLAS 2013, Orlando, FL; http://goo.gl/gzXUr.


4. Albert, K.J.; Bradshaw, J.T. et al. Verifying liquid handler performance for complex or non-aqueous reagents: a new approach. J. Assoc. for Lab. Automation Aug 2006, 11 (4), 172–80.


Rachel Parshley is a Product Development Sci- entist; John Thomas Bradshaw, Ph.D., is a Senior Development Scientist; and Karl Olson is a Pro- duction Chemist, Artel, Inc., 25 Bradley Dr., Westbrook, ME 04092, U.S.A.; tel.: 207-854-0860; fax: 207-854-0867; e-mail: info@artel-usa.com; www.artel-usa.com. This article is based on a poster presented at SLAS 2013, January 12–16, 2013, Orlando, FL, U.S.A.


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