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Focus 30 Years of PCR


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E Players in the beautiful history of PCR


If there were one technology synonymous with the molecular biology lab, it would have to be PCR. With many key applications making use of this transformative technology, celebrating its enormous impact and history becomes a challenge. In this special 30th


Anniversary Gem


section, two articles that have truly shaped the legacy of PCR are highlighted. First, a 1997 article from Wittwer et al. (reprinted on pg. 314) details the first use of SYBR Green in real-time PCR (qPCR) reaction monitoring. Tis is accompanied by a special commentary from Kent Vrana (pg. 312) on qPCR tools. Te second selection, available as a special reprint at www. biotechniques. com, comes from Sykes et al. (1992), who provide the first description of limiting dilution PCR—a precursor of digital PCR. We hope you enjoy revisiting these milestones in the history of PCR.


Vol. 54 | No. 6 | 2013 311 INTRODUCTION I


311 Method Evolution: Players in the beautiful history of PCR The Editors doi 10.2144/000114040


SPECIAL COMMENTARY


312 “Making it Real” Time Kent E. Vrana doi 10.2144/000114042


REPORT


314 Continuous fluorescence monitoring of rapid cycle DNA amplification Carl T. Wittwer, Mark G. Herrmann, Alan A. Moss, and Randy P. Rasmussen doi 10.2144/000114043


Quantitation of targets for PCR by use of limiting dilution P.J. Sykes, S.H. Neoh, M.J. Brisco, E. Hughes, J. Condon, A.A. Morley Available online at www.biotechniques.com


BioTechniques 54:311 (BioTechniques Focus: 30 Years of BioTechniques June 2013) doi 10.2144/000114040


www.BioTechniques.com


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