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Contents BIOFEEDBACK
228 [Letter to the Editor] In vitro use of free fatty acids bound to albumin: A comparison of protocols Ana F Oliveira, Daniel A Cunha, Laurence Ladriere, Mariana Igoillo-Esteve, Marco Bugliani, Piero
Marchetti, and Miriam Cnop doi 10.2144/000114285
REPORT
234 Non-destructive high-throughput DNA extraction and genotyping methods for cotton seeds and seedlings Xiuting Zheng, Kevin A. Hoegenauer, Andrea B. V. Maeda, Fei
Wang, David M. Stelly, Robert L. Nichols, and Don C. Jones doi 10.2144/000114286
244 Rapid quantification of microRNAs in plasma using a fast real-time PCR system William John Andrews, Eoin Daniel Brown, Margaret
Dellett, Ruth Esther Hogg, and David Arthur Simpson doi 10.2144/000114287
BENCHMARKS
253 Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencing Kaori Tatsumi, Osamu Nishimura, Kazu Itomi, Chiharu
Tanegashima, and Shigehiro Kuraku doi 10.2144/000114288
258 Influence of cell growth conditions and medium composition on EGFP photostability in live cells Anastasia V. Mamontova, Alexey M. Bogdanov, and
Konstantin A. Lukyanov doi 10.2144/000114289
262 A simple modification to the luminometric methylation assay to control for the effects of DNA fragmentation Elif Aysimi Duman, Skirmantas Kriaucionis, John J.
Dunn, Eli Hatchwell doi 10.2144/000114290
Vol. 58 | No. 5 | 2015 211
223 FEATURES
213 From the Editor - Making the best comparisons doi 10.2144/000114281
215 BioSpotlight - Fade resistant culturing
- Finding that better mate doi 10.2144/000114282
215 Citations
- Visualizing proteome-level metabolic dynamics - Tissue-specific genome editing in zebrafish doi 10.2144/000114282
217 Tech News
- Guiding our PCR experiments doi 10.2144/000114283
223 Special Feature
- CRISPR/Cas faces the bioethics spotlight doi 10.2144/000114284
272 New Products 273 Index to Advertisers
Sponsored Articles
266 Best practices for validating microarray expression data using VeriQuest® qPCR Master Mixes from Affymetrix
268 Evaluating EGFR Genotyping using HDx™ Reference Standards
270 Droplet Digital™ PCR: Multiplex Detection of KRAS Mutations in Formalin-Fixed, Paraffin-Embedded Colorectal Cancer Samples
Cover: “Tiki Goddess” trichrome stained frontal baby mouse tissue section. Image courtesy of George McNamara, L.J.N. Cooper Lab, M.D. Anderson Cancer Center, Houston, TX. Cover design by Genevieve McCarthy.
www.BioTechniques.com
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