This page contains a Flash digital edition of a book.
For more information, visit www.Q5PCR.com


Fidelity at its finest.


Q5® High-Fidelity


DNA Polymerase Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and performance. With the highest fidelity amplification available (>100X higher than Taq and 2X higher than Thermo Scientific®


Phusion® ), Q5 DNA Polymerase results


in ultra-low error rates. Its unique buffer system provides superior performance for a broad range of amplicons, regardless of GC content. Available in master mix and hot start formulations, Q5 DNA Polymerase represents the finest in fidelity.


Mandarin Ducks (Aix galericulata) are frequently featured in Chinese art and are regarded as a symbol of fidelity.


Robust amplification even with high GC amplicons 60% GC


78% GC bp L A B C


4900 2900 1900


1100 700 500


300


Amplification of two human genomic amplicons of mid to high GC content. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip®


manufacturer’s recommendations. For the 78% GC amplicon, GC Buffers or enhancers were used when supplied with the polymerase.


A = Q5 High-Fidelity DNA Polymerase (NEB) B = Phusion High-Fidelity DNA Polymerase (NEB) C = Company E D = Company A


PHUSION®


LABCHIP® NEBNEXT®


THERMO SCIENTIFIC® Phusion®


is a registered trademark and property of Thermo Fisher Scientific. is a registered trademark of Thermo Fisher Scientific.


DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. is a registered trademark of Caliper Life Sciences, part of Perkin Elmer, Inc. and Q5®


are registered trademarks of New England Biolabs, Inc. analysis. All polymerases were cycled according to D A B C D


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68