Contents BioTechniques
Special FocuS: 30 yearS oF BioTechniques
commentary
312 “Making it real” time Kent E. Vrana doi 10.2144/000114042
Special “Gem” article
314 Continuous fluorescence monitoring of rapid cycle DNA amplification Carl T. Wittwer, Mark G. Herrmann, Alan A. Moss, and Randy P. Rasmussen doi 10.2144/000114043
reportS
321 Capturing protein-coding genes across highly divergent species Chenhong Li, Michael Hofeiter, Nicolas Straube, Shannon Corrigan, and Gavin J.P. Naylor doi 10.2144/000114039
June 2013, Volume 54, Number 6 Features
327 Digital PCR to determine the number of transcripts from single neurons aſter patch-clamp recording Nóra Faragó, Ágnes K. Kocsis, Sándor Lovas, Gábor Molnár, Eszter Boldog, Márton Rózsa, Viktor Szemenyei, Enikő Vámos, Lajos I. Nagy, Gábor Tamás, and László G. Puskás doi 10.2144/000114029
337 Production of single-stranded DNAs by self-cleavage of rolling- circle amplification products Hongzhou Gu and Ronald R. Breaker doi 10.2144/000114009
BenchmarkS
345 Development of a high density hemagglutinin protein microarray to determine the breadth of influenza antibody responses Anthony L. Desbien, Neal Van Hoeven, Steven J. Reed, Allen C. Casey, John D. Laurance, Susan L. Baldwin, Malcolm S. Duthie, Steven G. Reed, and Darrick Carter doi 10.2144/ 000114041
293 From the Editor - Trends and traditions doi 10.2144/000114033
295 BioSpotlight - Gene capture touches down - Going digital doi 10.2144/000114034
297 Citations - TALEN scout - Disruptive technology doi 10.2144/000114035
299 Scientists - Revealing the mystery doi 10.2144/000114036
301 Tech News - Visiting “Noncodarnia” doi 10.2144/000114037
307 Troubleshooting Forum - Live cell imaging doi 10.2144/000114038
349 New Products 349 Index to Advertisers
Vol. 54 | No. 6 | 2013 Composite design based on electrophoresis images of 100mer ssDNA ladder generated using incomplete
deoxyribozyme self-cleavage. Image courtesy of H. Gu and R.R. Breaker. Design by Genevieve McCarthy. 287
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