Workflow Solutions for DNA Methylation Analysis
Promega offers cutting-edge, effective and flexible solutions to streamline your DNA methylation analysis workflow.
• Compatibility: Instruments and chemistries designed to work together and provide higher throughput and greater reproducibility
• Confidence: Consistent, high-quality reagents from a trusted manufacturer from start to finish
• Flexibility: Maximize your sample diversity upstream and downstream
DNA Methyla�on
Purification & Quantification ReliaPrep™
FFPE gDNA Purification– Brain DNA
Earlier Cq Values
Competitor Purification– Brain DNA
Bisulfite Conversion
Downstream Analysis Less Fragmented DNA gives earlier Cq
1.359 13.359 25.359 37.359 49.359 61.359
values
MethylEdge™ System
Cq
Control Converted MethylEdge Zymo NEB
30.0 0.07+ 23.7 0.07+ 23.1 0.15+ 22.8 0.17+ 28.2 & 1.21
– – – –
Tm
89.9 0.07+ 82.2 0.07+ 82.2 0.06+ 82.2 0.12+ 82.5 & 0.23
– – – –
Competing Conversion System
5 10 15 20 Cycles 25 30 35 40
Promega offers DNA purification chemistries and automation platforms for variable sample sizes and throughput needs as well as fast, sensitive and fluorescence- and PCR-based assays for accurate DNA quantitation.
The MethylEdge™ Bisulfite Conversion System offers efficient bisulfite conversion and cleanup of DNA from a variety of sources in less than 2 hours, with reduced template fragmentation.
Whether you need endpoint or real-time solutions, amplify your bisulfite-converted DNA with the most robust, reliable amplification tools on the market.
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Start simplifying your workflow with solutions designed to work together:
www.promega.com /dna_methylation
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