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Workflow Solutions for DNA Methylation Analysis


Promega offers cutting-edge, effective and flexible solutions to streamline your DNA methylation analysis workflow.


• Compatibility: Instruments and chemistries designed to work together and provide higher throughput and greater reproducibility


• Confidence: Consistent, high-quality reagents from a trusted manufacturer from start to finish


• Flexibility: Maximize your sample diversity upstream and downstream


DNA Methyla�on


Purification & Quantification ReliaPrep™


FFPE gDNA Purification– Brain DNA


Earlier Cq Values


Competitor Purification– Brain DNA


Bisulfite Conversion


Downstream Analysis Less Fragmented DNA gives earlier Cq


1.359 13.359 25.359 37.359 49.359 61.359


values


MethylEdge™ System


Cq


Control Converted MethylEdge Zymo NEB


30.0 0.07+ 23.7 0.07+ 23.1 0.15+ 22.8 0.17+ 28.2 & 1.21


– – – –


Tm


89.9 0.07+ 82.2 0.07+ 82.2 0.06+ 82.2 0.12+ 82.5 & 0.23


– – – –


Competing Conversion System


5 10 15 20 Cycles 25 30 35 40


Promega offers DNA purification chemistries and automation platforms for variable sample sizes and throughput needs as well as fast, sensitive and fluorescence- and PCR-based assays for accurate DNA quantitation.


The MethylEdge™ Bisulfite Conversion System offers efficient bisulfite conversion and cleanup of DNA from a variety of sources in less than 2 hours, with reduced template fragmentation.


Whether you need endpoint or real-time solutions, amplify your bisulfite-converted DNA with the most robust, reliable amplification tools on the market.


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Start simplifying your workflow with solutions designed to work together: www.promega.com /dna_methylation


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10139TC


Fluorescence (483-533)


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