ANALYTICAL AND LABORATORY EQUIPMENT 19


Fig. 4. A paired student’s T test was used to determine p values (95% confidence, N = 23). The direct-volume measurement method provides the least variability between replicate cell counts (average CV values: direct-volume measurement performed on the Accuri C6=2.10%; counting bead method =2.94%; hemacytometer =19.51%)3


.


traditional laminar flow fluidics and direct volume measurement capabilities is equally accurate and more precise, than either hemacytometer or counting bead methods for determining cell concentration3


. Te most precise


measurement was obtained by direct volume measurement with the flow cytometer (average CV


as chloroplasts. With over 6 decades of digital signal linearity, the Accuri C6 is the first flow cytometer to cover the full biological range of flowering plant genome sizes from 0.32 to 80.9


Fig. 3. Homogenates from Arabidopsis thaliana root tissues. A) Biparametric contour plot of FL2-A vs FL3-A fluorescence emission. B) Enlargement of the square region-of-interest (R1) containing the nuclei. C) Uniparametric histogram of FL2-A fluorescence, gated on region P1 of Panel B.


picograms in a single run (Fig. 3)2 .


Cell Counting A recent study explored three cell counting applications for viability determination for cultured cell lines, platelet counts in whole, unlysed human peripheral blood samples, and B- and T-cell concentrations in human peripheral blood. Te study showed that a flow cytometer with


References: 1


= 2.1%), with p values of 0.002 and 0.010 when compared to the hemacytometer and counting bead methods, respectively (Fig. 4).


Enter 19 or ✔ at www.scientistlive.com/elab MaryAnn Labant is with Accuri


Cytometers, Ann Arbor, MI, USA. www.AccuriCytometers.com


Vines, A. Blanco Fernández and G. McBean (2009). A Flow Cytometric Method for Continuous Measurement of Intracellular


Calcium. Irish Flow Cytometry Meeting Poster. 2


Galbraith DW (2009). Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described


angiosperm 2C values. Cytometry 75A:692-698. 3


Rogers C., Dinkelmann M., Bair N., Rich C., Howes G., Eckert B. (2009) Comparison of Tree Meth ods for the Assessment of Cell Phenotype, Viability, and Concentration in Cultures and Peripheral Blood. ASCB Poster.


www.scientistlive.com


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36